微囊藻毒素测定中产生的干扰及排除  

Elimination of interferences in the determination of trace level microcystins

作　　者：杨再荣[1,2] 董泽琴 张琳[1,2] 张维[1,3] 

机构地区：[1]贵州省环境科学研究设计院,贵州贵阳550002 [2]贵州师范大学地理与环境科学学院,贵州贵阳550001 [3]贵州省水污染控制与资源化技术研究重点实验室,贵州贵阳550002

出　　处：《环境污染与防治》2009年第5期36-39,43,共5页Environmental Pollution & Control

基　　金：贵州省社会发展科技攻关项目(黔科合S字[2007]1007号);贵州省优秀科技教育人才省长专项资金资助项目(黔省专合字[2007]108号);贵州省高层次人才科研条件特助经费资助项目(TZJF-2007-15)

摘　　要：采用固相萃取—高效液相色谱法定性、定量测定水中微囊藻毒素,对水样富集管(观赏鱼用氧气塑料管、橡胶管、医用乳胶管和硅胶管)、塑料材质(聚丙烯离心管等)、针式过滤器(水系、有机系针式过滤器)产生的干扰和吸附现象进行了深入探索。结果表明,观赏鱼用氧气塑料管对微囊藻毒素测定会产生严重的正干扰,在流动相甲醇∶磷酸盐缓冲溶液(体积比)为50∶50时,干扰得以排除;聚丙烯离心管在微囊藻毒素投加量较低时,对微囊藻毒素吸附率较高,造成回收率偏低;不同的针式过滤器对溶解在不同体积分数的甲醇溶液中的微囊藻毒素有明显不同的吸附能力。SPE-HPLC is commonly employed for qualitative and quantitative analysis of trace level microcystins in water. HPLC diagrams of the blank and microcystins solutions with and without filtration were utilized to determine the analytical interferences and microcystins adsorption on the sample enrichment tubing, plastic tube and stylus filter. The plastic tubings for sample enrichment produced positive interferences which were eliminated by the use of a mobile phase consisted of equal volumes of methanol and the phosphate buffer solution, mierocystins was adsorbed on the plastic centrifugation tubes causing a lower than expected recovery. Adsorption of microeystins in methanol solutions was dependent on the solution composition and the material of the stylus filters.

关 键 词：高效液相色谱 微囊藻毒素 干扰 吸附 

分 类 号：X524[环境科学与工程—环境工程]