一种快速、有效、经济的提取酵母质粒的方法  

One Fast and Economical Isolation Method of Plasmid from Yeast

作　　者：熊福银[1] 林艳丽[1] 吴晓洁[1] 周艳荣[1] 邓继先[1] 陈红星[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2009年第3期386-387,共2页Letters in Biotechnology

摘　　要：目的:建立一种经济有效、快速简便、稳定的提取酵母质粒的方法。方法:用葡糖苷酸酶消化酵母细胞壁以获取原生质体,然后采用碱裂解法裂解原生质体以获得质粒。结果:与采用商品化离心柱法试剂盒所提取的质粒相比,用该法获取的酵母质粒在PCR分析及转化效果方面没有差异。结论:建立了一种经济有效、快速简便、稳定的提取酵母质粒的方法。Objective： To establish a fast, stable and economical isolation method of plasmid from yeast. Methods： Add β-glucuronidase solution to the resuspended yeast cells. Mix well and incubate at 37℃ for 1/2 to 2 hours. Add lysis solution to the spheroplast pellet but do not resuspend. Then add precipitation solution and centrifuge. Transfer the supernatant carefully to a fresh microcentrifuge tube and precipitate the plasmid by ethanol. Results： Compared with commercial yeast plasmid isolation kit, the efficiency and stability was no significant difference between the two methods. Conclusion： We demonstrate the isolation of plasmid from yeast by β-glucuronidase treatment is efficiency and fast. It may be used as a regular method in laboratory.

关 键 词：酵母质粒 酶消化法 

分 类 号：Q78[生物学—分子生物学]