拉曼镊子在生物单细胞中的应用与进展  

Application and Progress of Raman Tweezers in Single Cells

作　　者：艾敏[1,2] 刘军贤[1] 黄庶识[2] 王桂文[2] 陈秀丽[1,2] 陈植成[1,2] 姚辉璐 

机构地区：[1]广西师范大学物理与电子工程学院,桂林541004 [2]广西科学院生物物理实验室,南宁530003

出　　处：《分析化学》2009年第5期758-763,共6页Chinese Journal of Analytical Chemistry

基　　金：广西科学基金(Nos.0575027,0718007b-30)项目资助

摘　　要：拉曼镊子(Raman tweezers)是将激光光镊(Optical tweezers)与显微拉曼光谱(Raman spectroscopy)结合的光学技术,可以在接近自然状态下研究单个生物细胞或细胞器。因其有无直接接触、无损伤、快速识别、实时追踪等特点,广泛用于生物细胞的识别、探测、筛选等。研究显示,拉曼镊子在微观生物研究的应用中,可提高拉曼光谱的信噪比,也能实现生化动力学过程的实时跟踪,从而能深刻了解细胞内生物大分子的活动规律。本文着重介绍了拉曼镊子的起源、原理及其在单细胞中的应用以及展望。Raman tweezers is a new optical technique coupled laser tweezers and Raman spectroscopy, which has the capability of studying single biological ceils or organelles in nearly-natured solution. Due to its characteristics of non-contact, non-invasion, fast identification and real-time detection, it can be used in the application of improving the Signal Noise Ratio of Raman spectroscopy, optimizing the detection and sorting of single ceils or organelles, real-time detection of biochemistry dynamics process widely. And the activity and mechanisms of bio-molecules can be understood deeply. The source, principle of Raman tweezers and its optical application in biology are depicted and reviewed particularly.

关 键 词：拉曼光谱 光镊 单细胞 拉曼镊子 评述 

分 类 号：Q503[生物学—生物化学]