水痘-带状疱疹病毒基因特征分析  

Gene characteristics of varicella-zoster virus

作　　者：李崇山[1] 鲁礼瑞 陆菁[1] 翁康生[1] 汤素文[1] 丁晓光[1] 刘敏勇[1] 李云逸[1] 胡家瑜[1] 

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]上海市技术交易所

出　　处：《疾病监测》2009年第3期168-171,共4页Disease Surveillance

摘　　要：目的对引起上海市某小学水痘暴发的水痘-带状疱疹病毒进行基因特征分析。方法对采集到的患者疱疹液用MRC-5细胞进行病毒分离,用水痘-带状疱疹病毒标准血清鉴定病毒分离株,并通过聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)和序列测定对分离到的病毒进行基因特征分析。结果从采集的5份疱疹液标本中均分离到导致细胞病变的病毒,经水痘-带状疱疹病毒标准血清中和鉴定,证实为水痘-带状疱疹病毒;对分离病毒的ORF22、38、54、62、68等不同区段进行PCR-RFLP或序列测定结果显示:所有分离到的水痘-带状疱疹病毒均为J基因型野毒株(BglⅠ阳性,PstⅠ阳性,SmaⅠ阳性);糖蛋白E中3B3抗原决定簇的第150位氨基酸未发现类似北美VZV-MSP株或VZV-BC株的碱基突变。结论此次水痘暴发是由J基因型水痘-带状疱疹病毒野病毒引起。有41.1%的患者曾经接种过水痘疫苗,初免失败或者抗体水平下降可能是造成水痘突破的原因。因此有必要对病毒基因特征及水痘疫苗免疫策略进行进一步的研究。Objective To analyze the gene characteristics of varicella-zoster virus （VZV） that caused an outbreak of chicken-pox in a primary school in Shanghai in 2007. Methods The virus was isolated in vesicular fluid collected from 5 patients by using MRC-5 cells and identified by standard serum for VZV. PCR-RFLP and nucleotide sequencing were performed to characterize the gene of isolated virus. Results The virus that can cause cytopathic effect （CPE） of MRC- 5 cells was isolated from 5 vesicular fluid samples and identified as VZV by the neutralization test with standard serum for VZV. PCR-RFLP and sequencing performed on fragment ORF22, 38, 54, 62 and 68 of VZV genome showed that all the isolated VZV were wild strains of genotype J （BglⅠ ＋ ,PstⅠ ＋ ,SinaⅠ ＋ ）. VZV-MSP or VZV-BC-like genetic mutation in antigenic determinant 3B3 of glycoprotein E was not found in these VZV isolates. Conclusion This outbreak of chickenpox was caused by wild strain of VZV （genotype J）. Among the patients, 41.1% had received varicella vaccine. Failure of initial immunization or decrease of antibody level might account for this chicken-pox outbreak. So it is necessary to conduct further study on the gene characteristics of VZV and varicella vaccine vaccination strategy.

关 键 词：水痘-带状疱疹病毒 暴发 免疫策略 J基因型 

分 类 号：R450[医药卫生—治疗学]