ADVIA2120全自动血球分析系统HGB、RBC及WBC线性范围的测定与评价  

ADVIA2120 Automatic blood cell analysis system HGB,RBC and WBC determination of linear range and evaluation

作　　者：张晓耕[1] 王培昌[1] 刘辰庚[1] 郭宏林[1] 张丽春[1] 黄花[1] 孙颖[1] 

机构地区：[1]首都医科大学宣武医院检验科,北京100053

出　　处：《中国实验诊断学》2009年第5期658-661,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的测定并分析Bayer ADVIA 2120全自动血细胞分析系统HGB、RBC、WBC线性范围,以评价该检测方法的性能。方法以健康人混合抗凝全血制备高值样本,高值样本WBC及RBC赋值采用细胞计数法,HGB赋值采用氰化高铁血红蛋白法,高值样本用同型非溶血血浆稀释,在校准后的血细胞分析系统上依低高及高低顺序分别行3次测定,以预期值与实测值做直线相关与回归分析。结果HGB线性范围为0-280 g/L,相关系数γ=0.998,单侧t检验P<0.005;RBC线性范围为0-7.99×1012/L,相关系数γ=0.994,P<0.001;WBC线性范围为0-521.3×109/L,相关系数γ=0.985,P<0.05。结论结论本系统HGB、RBC、WBC线性区间评估值比该仪器生产厂家自测值略宽,提示上述项目的可报告范围应宽于厂家设定的范围。Objective To investigate the finear ranges of HGB, RBC, WBC by automatic blood cell analytical system, Bayer ADVIA2120 so as to appraise the performance of the measured method.Methods the high-value samples were prepared with mixed anticoagulant blood in health people. The values of WBC and RBC were counted by cell counting method;the values of HGB were measured with cyanide methemoglobin method, the samples were diluted by the non-hemolytic plasma. The samples were measured 3 times respectively in accordance with the level of high and low order. The expected values do linear correlation and regression analysis with the measured values. Results The linear range of HGB is 0- 280 g/L, the correlation coefficient is 0.998, unilateral T test is P 〈 0.05 ; the linear range of RBC is 0 - 7.99×10^12/L, the correlation coefficient is 0. 994, P 〈 0. 001 ; the hnear range of WBC is 0 - 521.3×10^9/L. the correlation coefficient is 0. 985, P 〈 0.05. Conclusion The linear ranges of HGB, RBC, WBC which we measured are slightly wider than the ranges the manufactory offered. This suggests the reported ranges of subjects above should be broader than the scope of factory settings.

关 键 词：ADVIA 2120 血细胞分析系统 线性范围 细胞 血红蛋白 

分 类 号：R446.1[医药卫生—诊断学]