复方中药对人宫颈癌Hela细胞生长和凋亡的影响  

Effects of complex prescription of traditional Chinese medicine on the growth of HeLa cells in Vitro

作　　者：谭忠伟[1] 江振友[2] 岳磊[2] 邹林[2] 施珊珊[2] 肖京中 

机构地区：[1]江门市妇幼保健院检验科,529000 [2]暨南大学医学院微生物学与免疫学教研室,510632 [3]广州葆盛科技开发有限公司,510000

出　　处：《国际医药卫生导报》2009年第9期5-8,共4页International Medicine and Health Guidance News

基　　金：广东省科技计划项目课题（NO：83057）,广东省中医药局建设中医药强省科研课题（NO.1060117）

摘　　要：目的研究观察复方中药提取物（葆盛口服液）对体外培养的人宫颈癌Hela细胞生长的影响及其作用机制。方法以人宫颈癌Hela细胞为研究对象，用MTT法观察葆盛口服液对Hela细胞生长的影响，以肝细胞株7701作为比较；流式细胞仪分析Hela细胞凋亡以及凋亡基因Fas的表达情况，以顺铂作为对照。结果葆盛口服液对Hela细胞有较强的体外增殖抑制作用，流式细胞术发现葆盛口服液能促进Fas表达，诱导Hela细胞凋亡。结论葆盛口服液诱导细胞凋亡，在抗肿瘤作用中可能具有重要意义。Objective To investigate the influence of the traditional Chinese medicine extract （Baosheng Oral Liquid ） on the growth of human cell line Hela in vitro and its probable mechanism. Methods The inhibition of Baosheng Oral Liquid on Hela cells and human normal liver line 7701 cells proliferation was detected by MTT method, cell apoptosis and expression of apoptosis gene fas were further analyzed with flow cytometry in which antitumor drug Cisplatin was employed as control. Results Baosheng Oral Liquid remarkably inhibited the growth of Hela cells. Flow cytometric analysis showed that apoptosis of the tumor cells was induced and expression of Fas was enhanced. Conclusion apoptosis may play an important role in the anti-tumor effect of Baosheng Oral Liquid.

关 键 词：复方中药 细胞凋亡 FAS基因 

分 类 号：R28[医药卫生—中药学]