桥联型双核金属配合物[(Phen)_2Fe-Im-Fe(Phen)_2](BF_4)_5的合成、表征及其超氧化物歧化酶模拟活性测定  

Synthesis,characterization of a new binuclear metal complex with imidazole bridge and determination of its mimic enzyme activity of superoxide dismutase

作　　者：曹惠平[1] 李彦岗[1] 常云珍[1] 高飞(指导)[1] 

机构地区：[1]山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《化学工程师》2009年第5期16-20,共5页Chemical Engineer

基　　金：山西省基础研究资助项目(2006011018)

摘　　要：本文合成了一种新的超氧化物歧化酶模拟化合物[(Phen)2Fe-Im-Fe(Phen)2](BF4)5,通过红外光谱、紫外光谱及摩尔电导测定对其结构进行了表征;采用改进的邻苯三酚自氧化法,在体系的最大吸收波长(λmax=320nm)处检测了其超氧化物歧化酶模拟活性。结果表明:模拟化合物在25℃、0.1mo·lL-1Tris-HCl缓冲溶液(pH=8.2)条件下,具有明显抑制O-·2活性的作用,是较好的超氧化物歧化酶模拟物,其IC50值为24.34mg·L-1。A new superoxide dismutase mimetic complex, [（Phen）2Fe-Im-Fe（Phen）2] （BF4）5 has been synthesized and characterized by IR, UV spectroscopy and mole conductance. Using a modified method of self-oxidization of pyrogallol, we investigated the SOD mimic activity of the dinuclear complex （λmax = 320 nm）. The result showed that the new complex was able to inhibit the O2· activity evidently at 25℃ in the buffer of 0.1 mol·L-1 Tris-HCl, with pH8.2 and it might qualify as a candidate of better superoxide dismutase simulacrum, with a IC50 value to be 24.34 mg· L-1.

关 键 词：双核配合物 合成 表征 超氧化物歧化酶模拟活性 

分 类 号：O629.8[理学—有机化学]