中华鳖抗菌肽Hepcidin成熟肽基因的克隆及同源性的初步研究  

Molecular Cloning and Characterization of Hepcidin Mature Peptide from Soft-shelled Turtle and Preliminary Study on Its Homology

作　　者：王海静[1] 慕玉[1] 赵红艳[1] 李寒鸣[1] 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016

出　　处：《河北师范大学学报（自然科学版）》2009年第3期387-392,共6页Journal of Hebei Normal University：Natural Science

基　　金：河北省科技攻关项目(067805003D)

摘　　要：采用同源克隆的方法,以用于扩增大菱鲆hepcidin成熟肽基因的引物,从中华鳖肝脏cDNA中扩增得到1条长78 bp的基因片段.初步判断其属于Hepcidin家族,与HAMP1具有较高的同源性,分析表明其为Hepcidin成熟肽.根据基因序列推断得到的成熟肽蛋白序列如下:QSHISLCRWCCNCCKANKGCGFCCKF.与其他物种的成熟肽进行比对,发现其与大菱鲆的成熟肽序列同源性达到100%,并构建了基于各物种Hepcidin前体肽的系统发育树,为研究物种进化提供了证据.A fragment of 78 bp is amplified from the soft-shelled turtle liver cDNA using homology-based cloning methods with primers used for amplification of turbot hepcidin mature peptide gene. Initial evaluation reveals that it belongs to hepcidin family,and having a high homology with HAMP1. Analysis indicates that it may be hepcidin mature peptide. The amino acid sequence of mature peptide deduced from the gene fragment sequence is QSISIISLCRWCCNCCKANKGCGFCCKF. After alignment by blast, the sequence shares 100 % homology with turbot. A phylogenetic tree, which may provide evidence for species evolution reaserch, is constructed based on the hepcidin propeptide alignment.

关 键 词：抗菌肽 HEPCIDIN 中华鳖 成熟肽 

分 类 号：Q785[生物学—分子生物学]