大白菜-结球甘蓝双单体异附加系的SSR鉴定及其特性  

Identification of Chinese Cabbage-Cabbage Double Alien Monosomic Addition Line by SSR and Studies on Its Characters

作　　者：顾爱侠[1] 王娟[1] 陈雪平[1] 罗双霞[1] 王彦华[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001

出　　处：《中国农业科学》2009年第5期1698-1704,共7页Scientia Agricultura Sinica

基　　金：河北省自然科学基金资助项目(C2005000222);河北农业大学将帅计划项目

摘　　要：【目的】筛选大白菜-结球甘蓝异附加系,利用SSR标记鉴定附加的外源染色体,并对异附加系特性进行研究。【方法】以大白菜-结球甘蓝异源三倍体(AAC)与二倍体大白菜(AA)回交一代的自交后代为试材,利用根尖染色体计数、甘蓝连锁群特异SSR标记等方法,筛选、鉴定大白菜-结球甘蓝异附加系。【结果】在BC1F2中筛选到2n=22的植株;该植株对位于甘蓝01连锁群的3对SSR引物和08连锁群的2对SSR引物的扩增结果与异源三倍体杂交种是一致的,而对位于其它7个连锁群的20对特异引物的扩增结果与大白菜一致,表明该植株附加的外源染色体与甘蓝的01、08连锁群相对应,为双单体异附加系;该双单体异附加系绝大多数花粉母细胞减数分裂终变期染色体构型为10Ⅱ+2Ⅰ,后期Ⅰ以11-11、10-12分离方式为主,后期Ⅱ产生了n=11配子。【结论】利用甘蓝连锁群相对于大白菜特异的SSR标记可以准确鉴定大白菜-结球甘蓝异附加系;获得了附加甘蓝01、08连锁群的大白菜双单体异附加系,为进一步合成附加甘蓝01或08连锁群大白菜-结球甘蓝单体和双体异附加系奠定基础。[Objective] Chinese cabbage-cabbage alien addition line was screened, alien chromosomes introduced into Chinese cabbage were identified by SSR, and the characters of the alien addition line were further analyzed. [Method] The BC1F2 progenies derived from allotriploid hybrid （AAC） and diploid Chinese cabbage （AA） were used as materials, by using analysis of the root tip cell chromosome number and linkage group-specific SSR markers from linkage groups of cabbage, the Chinese cabbage-cabbage alien addition line was selected and identified. [Result] A plant （2n=22） was obtained. Amplification results of 3 pairs of primers assigned linkage group 01 and 2 pairs of primers assigned 08 linkage group on this plant were the same as allotriploid hybrid, however, the amplification results of 20 pairs of primers assigned other 7 linkage groups were the same as Chinese cabbage. Therefore, the introduced cabbage chromosomes in this plant were grouped into linkage groups 01 and 08 of cabbage, which was double alien monosomic addition line. Ten bivalents and two univalents were observed at the stage of diakinesis in most of double alien monosomic addition line＇s PMCs, 11-11 and 10-12 distributions were observed at anaphase I in most of PMCs, and the gametes with n= 11 were observed at anaphase Ⅱ. [ Conclusion ] Chinese cabbage-cabbage addition alien line could be exactly identified by specific SSR markers for cabbage compared with Chinese cabbage. And Chinese cabbage-cabbage double alien monosomic addition line added linkage groups 01 and 08 of cabbage was obtained, which laid a foundation for further obtaining the Chinese cabbage-cabbage monosomic alien addition lines and disomic alien addition lines added linkage group 01 or 08 of cabbage.

关 键 词：大白菜 结球甘蓝 异附加系 连锁群特异SSR 特性 

分 类 号：S634.1[农业科学—蔬菜学]