椰扁甲啮小蜂寄生后椰心叶甲蛹血淋巴蛋白质双向电泳图谱分析  

Analysis of hemolymph protein patterns from Brontispa longissima(Coleoptera:Chrysomelidae)pupae parasitized by Tetrastichus brontispae(Hymenoptera:Eulophidae)

作　　者：刘奎[1] 林健荣[2] 符悦冠[1] 彭正强[1] 金启安[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所、农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737 [2]华南农业大学动物科学学院,广州510642

出　　处：《昆虫学报》2009年第5期588-594,共7页Acta Entomologica Sinica

基　　金：农业部公益性项目(2004DIA4J012,2005DIB4J043);农业部成果转化项目(05EFN216900370);国家科技支撑项目(2006BAD08A18)

摘　　要：分析昆虫被寄生蜂寄生后血淋巴蛋白质组分及组分含量变化,从蛋白质水平分析寄生蜂寄生引起寄主昆虫的生理变化,有利于探索寄生蜂调控寄主的机理。以椰心叶甲Brontispa longissima和椰扁甲啮小蜂Tetrastichus brontispae为研究材料,采用蛋白质双向电泳技术及图像分析技术分析了椰心叶甲蛹被椰扁甲啮小蜂寄生后不同时期血淋巴蛋白质图谱及其变化情况。结果表明:寄生后0.5,1,2,3和4d,被寄生蛹血淋巴共检测到的蛋白斑点数分别为982,926,712,636和680个,其中特异性蛋白斑点的数量分别为650,400,336,229和150个;同期未被寄生蛹血淋巴共检测到的到的蛋白斑点数分别为645,817,640,873和940个,其中特异性蛋白斑点的数量分别为313,291,264,466和410个;被寄生蛹和同期未被寄生蛹能匹配的蛋白斑点分别为332,526,376,407和530对。被寄生蛹血淋巴蛋白斑点数量的异常变化表明寄主的生理功能受到了严重的影响。The comparison of hemolymph protein composition and content between the parasitized and unparasitized host will contribute to explore the regulating mechanism of parasitization. Using Brontispa longissima and Tetrastichus brontispae as experimental insects, the change of protein patterns from the parasitized and unparasitized Brontispa longissima pupae at different stages were analyzed using two dimensional gel electrophoresis （2-DE） and image analysis system. The results showed that at 0.5, 1, 2, 3 and 4 d after parasitization, 982, 926, 712, 636 and 680 protein spots were detected in the parasitized pupae respectively, of which 650, 400, 336, 229 and 150 were the specific protein spots; there were a total of 645, 817, 640, 873 and 940 protein spots in the unparasitized pupae, respectively, of which 313, 291,264,466 and 410 were the specific protein spots. Comparison result showed that 332,526,376,407 and 530 pairs were matched in both the parasitized and unparasitized pupae at 0.5, 1, 2, 3 and 4 d after parasitization, respectively. The irregular changes in protein patterns suggest that the physiological function of host is affected seriously by the parasitism.

关 键 词：椰扁甲啮小蜂 椰心叶甲 寄生 血淋巴蛋白质 双向电泳 图谱分析 

分 类 号：Q966[生物学—昆虫学]