尿液HCMV-DNA和血清HCMV-IgM抗体诊断人巨细胞病毒感染的价值  

Dingnostic significance of detecting urine HCMN-DNA and serum HCMV-IgM

作　　者：齐莹[1] 何蓉[1] 马艳萍[1] 孙峥嵘[1] 刘庆[1] 王继东[1] 吉耀华[1] 阮强[1] 

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《山东医药》2009年第19期18-19,共2页Shandong Medical Journal

摘　　要：目的提高人巨细胞病毒(HCMV)感染的诊断水平。方法对960例临床疑诊HCMV感染患儿分别应用FQ-PCR、CLIA法检测尿液HCMV-DNA及血清HCMV-IgM抗体水平。结果尿液HCMV-DNA阳性478例,HC-MV-IgM抗体阳性206例;二者均阳性191例。478例尿液HCMV-DNA阳性者中,血清IgM抗体阳性HCMV-DNA拷贝数对数均值高于阴性者(P<0.01)。结论尿液HCMV-DNA定量和血清HCMV-IgM抗体联合检测有助于HCMV感染的诊断。Objective To improve the diagnostic level of HCMV infection. Methods 960 suspected cases with HCMV infection were selected , and the urine HCMV-DNA and serum HCMV-IgM of which were detected by Fluorescent Quantitative PCR（FQ-PCR） and LIAISON Chemilumineseent Immanoassay （CLIA） respectively. Results 478 eases were positive for HCMV-DNA ,and 206 eases were positive for HCMV-IgM. 191 cases were positive for two indexes. The viral DNA load（logarithmic mean）in serum HCMV-IgM positive cases was higher than those in negative eases （P 〈 0. 01 ）. Conclusion Combined detection of urine HCMV-DNA and serum HCMV-IgM is benefical to the diagnosis of HCMV infection.

关 键 词：人巨细胞病毒 婴儿 荧光定量聚合酶链反应 化学发光免疫分析法 

分 类 号：R752.1[医药卫生—皮肤病学与性病学]