中国斗鸡高分子质量DNA的高效制备  

An Efficient Preparation of High Molecular Weight DNA from Chinese Cockfight

作　　者：潘春留[1] 闫晶[1] 余露露[1] 关伟军[2] 卿素珠[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《动物医学进展》2009年第5期24-27,共4页Progress In Veterinary Medicine

基　　金：国家自然科技资源平台建设项目(2005DKA21101)

摘　　要：采集斗鸡翅静脉血液,加ACD抗凝,离心收集血细胞,经细胞计数后,用低融点琼脂糖包埋血细胞,蛋白酶K消化,经脉冲电泳分离,获得了较纯净的720 kb以上的DNA。对大片段DNA进行限制性酶切,回收特定大小的DNA片段。利用该方法制备的高分子质量DNA,经酶切、回收的特定大小DNA片段可用于后续细菌人工染色体(BAC)文库的构建和基因组分析。The blood of Chinese cockfight was selected as our experimental material. The blood cells were collected by the means of centrifugation, embedded in the low melting-point agarose. After digestion with proteinase K and isolated by pulsed-field gel electrophoresis, pure HMW unclear DNA over 720 kb was isolated. Restriction enzyme was used to digest the HMW nuclear DNA in order to recovery special molecular weight nuclear DNA. The HMW nuclear DNA prepared and the special molecular weight nuclear DNA using this method was suitable for the construction of bacterial artificial chromosome library （BAC）and genome analysis of Chinese cockfight.

关 键 词：中国斗鸡 高分子质量DNA 脉冲凝胶电泳 

分 类 号：Q78[生物学—分子生物学] S858.3[农业科学—临床兽医学]