肾综合征出血热患者病毒特异性淋巴细胞的临床分析  

The clinical analyses of the Levels of virus-specific lymphocyte of hemorrhagic fever with renal syndrome

作　　者：王小芳[1] 谢明[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061

出　　处：《基层医学论坛》2009年第13期388-390,共3页The Medical Forum

摘　　要：目的通过检测外周血中分泌IFN-γ的淋巴细胞含量水平,探讨病毒特异性淋巴细胞在HFRS发病机制中的作用。方法对我院2008年2月-7月收治的31例肾综合征出血热患者用流式细胞仪对PMBC体外刺激后IFN-γ的淋巴细胞含量水平进行检测。结果发热期组、低血压期组、少尿期组、多尿期组和恢复期组含量相互间均有显著统计学差异(P<0.05),且发热期、少尿期和多尿期含量均与健康对照组有显著统计学差异(P<0.05)。结论HFRS发病机制尚未完全清楚,但动态观察不同病程HFRS患者淋巴细胞表达的消长规律可能会成为监测HFRS病情状态的一个新指标。Objective To explore the role of virus-specific lymphocyte played in the pathogenesis of hemorrhagic fever with renal syndrome （HFRS）by detecting the levels of virus-specific lymphocytes which secrete IFN-γ in peripheral blood of patients with HFRS. Methods In this study, 31 patients with HFRS were selected from inpatients during from 2008 February to July.They were detected the levels of hantavirus-specific lymphoeytes which secreted IFN- γ of peripheral blood mononuclearcells （PMBCs） by flow cytometry after the PMBCs were excited in vitro by hantavirus （HTNV）.Results The levels of HTNV-speeific lymphocyte in patients with HFRS in different groups, for example, the fever phase group, hypotension group ,oliguria stage group , diuresis stage and recovery group were significantly defferent （P〈0.05）. The level of virus-specific lynlphocytes in fever phase group, the oliguria phase group and the polyuria phase group were significantly higher than that in healthy controls （P〈0.05）.Conclusion The level of virus-specific lymphoeytes in patients with different stage may be a new mark for pathogenetic condition of HFRS.

关 键 词：肾综合征出血热 病毒特异性淋巴细胞 IFN-Γ 

分 类 号：R512.8[医药卫生—内科学]