异种抗磷脂抗体皮下注射致妊娠丢失大鼠模型的改良  

Improvement of rat model of fetal loss induced by human APA

作　　者：卫爱武[1] 宋艳丽[2] 成琼[3] 马存亮[4] 倪婷婷[2] 

机构地区：[1]河南中医学院第一附属医院妇产科,郑州450003 [2]河南中医学院研究生,郑州450008 [3]河南中医学院第一附属医院病理科,郑州450003 [4]河南中医学院第一附属医院检验科,郑州450003

出　　处：《郑州大学学报（医学版）》2009年第3期526-530,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省自然科学基金资助项目0511043700

摘　　要：目的:制备异种抗磷脂抗体皮下注射致妊娠丢失大鼠模型的改良方法。方法:将80只10周龄SD孕鼠随机分为A～H组共8组(每组各10只),分别于孕8d、孕12d背部多部位皮下注射25、50、75、100mg/(kg·d)抗心磷脂抗体(ACA,A、B、C、D组)或狼疮抗凝物(LA,E、F、G、H组)蛋白液,以注射健康人血清[75mg/(kg·d)]的SD孕鼠作为空白对照(10只),至孕15d处死后取材,计算各组孕鼠胚胎吸收率、胚胎质量,酶标仪测各组血清ACA的相对含量,以部分凝血酶原时间(APTT)来判定LA的活性。结果:造模后,孕鼠血清ACA、APTT、胚胎吸收率明显增加(P<0.05);胚胎质量明显降低(P<0.05);胎盘切片镜下见胎盘免疫复合物沉积、组织变性坏死、炎性细胞浸润、血管内纤维素凝结及淤血等病理现象;且均与抗体蛋白液剂量呈正相关,其中以注射75mg/(kg·d)ACA蛋白液或LA蛋白液造模最稳定。结论:LA可作为复制本动物模型所需免疫抗体来源之一;75mg/(kg·d)ACA蛋白液或LA蛋白液为本模型所需免疫抗体的稳定有效量;改良后动物模型符合抗磷脂抗体致妊娠丢失患者的生化、产科特点及病理变化。Aim ： To investigate the improved methods for setting up rat model of fetal loss induced by human APA. Methods：80 10-week-old SD pregnant rats were randomly divided into 5 groups： A - H and blank control group. On the 8th and 12th day of pregnancy, multi-site subcutaneous injection of the anticardiolipin antibody（ ACA ,Group A - D） or lupus anticoagulant （ LA,Group E -H） of protein solution on the back was used to every rat of Group A - H in the dosage of 25,50,75, and 100 mg/（kg · d）. Until the 15th day of pregnancy, they were killed for taking samples. Meanwhile, healthy human protein solution was taken as a blank control group. Absorption rate and weight of fetus were calculated ;75 mg/（kg·d） ACA level was measured by enzyme mark instrument and activities of LA determined by APTT. Results： Studying the rat model, we found that the number of ACA in serum and embryo absorption rate increased significantly （ P 〈 0.05 ） , and APTT prolonged, while embryonic weight decreased significantly （ P 〈 0.05 ）. The results of pathological observation showed some phenomena such as deposit of plaeenta immune complex, tissue degeneration and necrosis, inflam- matory cell infiltration, fibrin agglutinations and blood stasis in the vessel, etc, which were all positively correlated with dosages of antibody protein solution. In addition, 75 mg/（ kg · d） ACA protein solution or LA protein solution is the stable and effective dosage of immune antibody for the model. Conclusion ： LA can be one of the immune antibody sources to replicate the animal model; 75 mg/（ kg ·d） ACA protein solution or LA protein solution is the stable and effective immune antibody dosage for a model; the improved animal model conforms to the biological, obstetrical and pathological changes of fetus loss patients induced by APA.

关 键 词：抗磷脂抗体 妊娠丢失大鼠模型 改良研究 

分 类 号：R714.2[医药卫生—妇产科学] R392.12[医药卫生—临床医学]