体外染烟对V79细胞增殖和凋亡的影响  

Effects of Tobacco Smoke on Proliferation and Apoptosis of V79 Cells in vitro

作　　者：刘海云[1] 张素萍[1] 童建[1] 李建祥[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院卫生毒理学教研室,江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2009年第1期11-13,20,198,共5页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助资助项目(30671784)

摘　　要：目的研究体外香烟烟雾对V79细胞增殖和凋亡的影响。方法将V79细胞分为对照组、5%浓度组和20%浓度组进行染烟实验。染烟后检测细胞内ROS水平、细胞增殖活性、细胞周期及凋亡情况。结果5%浓度和20%浓度染烟组与对照组相比,V79细胞ROS水平均明显升高,且20%浓度组与5%浓度组相比ROS也明显升高,差异均有统计学意义(均P<0.05)。5%浓度和20%浓度染烟组与对照组相比,细胞增殖率、克隆率明显降低;G1期细胞百分比明显升高,G2期和S期细胞明显减少,差异均有统计学意义(均P<0.05)。20%浓度染烟组分别与对照组和5%浓度染烟组相比,细胞凋亡率均明显升高,差异有统计学意义(P<0.05)。结论香烟烟雾可能刺激了V79细胞内ROS的增多,影响细胞增殖和凋亡率。Objective To investigate the effects of tobacco smoke on proliferation and apoptosis of V79 cells. Methods V79 cells were divided into three groups： the control group, 5% group, 20% group. The level of ROS,the effect of cell growth inhibition,the cell cycle and apoptosis were assayed. Results The level of ROS in the 5% and 20% groups was significantly higher than that in the control group, and ROS in the 20% group was significantly higher than that in the 5% group （P〈0.05）; the cell growth rate and cloning rate in the 5% and 20% groups were significantly lower than that in the control group（P〈0.05）, the percentage of cells in G1 phase in the 5% and 20% groups was significantly higher, while G2 those in phase and S phase： were significantly lower than that in the control group. The apoptoticrates in the 20% group was significantly higher than those in the control group and the 5% group （P〈 0.05）. Conclusion Tobacco smoke may effet proliferation and apoptoticrates of V79 cells via stimulating the production of ROS in cells.

关 键 词：染烟 体外 细胞增殖 细胞凋亡 活性氧 

分 类 号：R332.2[医药卫生—人体生理学]