生理浓度铁调素对成骨细胞铁离子的影响  

Mechanism on Effect of Hepcidin out of Osteoblast on Iron Transportation

作　　者：赵东阳[1] 徐又佳[1] 张鹏[1] 郝彦明[1] 马勇[1] 刘虎[1] 

机构地区：[1]苏州大学附属第二医院骨科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2009年第1期36-37,58,197,共4页Suzhou University Journal of Medical Science

基　　金：江苏省自然科学基金资助项目(05KJB320125);江苏省医学重点人才资助项目(RC2007100);江苏省"六大人才高峰"资助项目(07-B-032)

摘　　要：目的研究生理浓度铁调素(Hepcidin)对人成骨细胞(hFOB1.19)内铁离子转运的影响。方法加入终浓度分别为100(A1组)、300(A2组)、600(A3组)nmol/L的Hepcidin,干预成骨细胞20h后在激光共聚焦显微镜(CLSM)下测量细胞内的铁离子荧光强度。结果细胞内铁离子荧光强度对照组(A0组)为5.23±1.54,A1组为20.74±4.90,A2组为21.65±6.55,A3组为22.32±6.91,与A0组相比,A1组、A2组、A3组荧光强度都明显升高,差异有统计学意义(P<0.05),而A1组、A2组、A3组之间的差异均无统计学意义(P>0.05)。结论Hepcidin具有升高成骨细胞(hFOB1.19)内铁离子的作用,但在100～600nmol/L剂量范围内,Hepcidin升高成骨细胞内的铁离子浓度无明显差异。Objective To study the Effect of Hepcidin of different concentration out of hFOB 1. 19 cells on iron transportation in the different time. Methods HFOB1.19 ceils were cultured and Hepcidin of different concentration was added into the experiment group：the A1 group（the final concentration was 100 nmol/L） A2 group（300 nmol/L） A3 group（600 nmol/L）, and the fluorescence intensity of iron was observed by confocal laser scanning microscope （CLSM） 20 hours later. Results The fluorescence intensity of iron ions in hFOB 1.19 in the control group was 5.23±1.54, while in the A1 group was 20.74± 4.90, the A2 group was 21.65±6.55, the A3 group was 22.32±6.91. The fluorescence intensity of iron ions of the three groups was significantly higher than that in the control group （P〈0.05）, while there was not significant difference among the three groups （P〉0.05）. Conclusion The iron concentration is increased significantly by adding Hepcidin out of hFOB 1.19 cells 20 hours later, but there is not significant difference is observed when the Hepcidin concentration is 100-600 nmol/L.

关 键 词：铁调素 铁离子 成骨细胞 骨质疏松 

分 类 号：R336[医药卫生—人体生理学]