^(188)Re标记丙氧鸟苷白蛋白纳米微球的制备  

Labeling Bovine Serum Albumin-Coated Ganciclovir Nanoparticles with ^(188)Re

作　　者：詹惠英[1] 唐秋莎[2] 汤月萍[1] 陈道桢[1] 

机构地区：[1]南京医科大学附属无锡妇幼保健院中心实验室,江苏无锡214002 [2]东南大学基础医学院病理与生理学系,江苏南京210009

出　　处：《苏州大学学报（医学版）》2009年第1期105-107,115,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30500601;30872999);江苏省自然科学基金资助项目(BK2007023);南京医科大学重点资助项目(06NMUZ044);无锡市医院管理中心重大技术资助项目(ZD0604)

摘　　要：目的制备188Re标记丙氧鸟苷(GCV)白蛋白纳米微球(188Re-GCV-BSA-NP)。方法通过蛋白交联固化的方法制备GCV-BSA-NP,并测定GCV-BSA-NP的体外释药特性;采用预锡化直接标记法对GCV-BSA-NP进行188Re标记,同时观察188Re-GCV-BSA-NP体外稳定性。结果GCV-BSA-NP为大小不等的规则性微球,微球直径不等,但多集中在250~290nm。GCV-BSA-NP总的标记率>90%,放化纯>95%,放射性比活度1.85×105MBq/μmol。标记物放置于37℃的PBS(pH7.4)溶液中,在24h时放化纯为95%。结论预锡化直接标记法简便快速,能够得到较高的标记物放化纯度,稳定性好,GCV-BSA-NP具有明显的缓释功能。Objective To prepare and characterize bovine serum albumin（BSA）-coated ganeiclovir （GCV）nanoparticles labeled with rhenium-188（^188Re）. Methods GCV-BSA-NP was prepared by a chemical cross-linking technique,and the release of the GCV from the nanosphere was measured.Directly labeled raRe to BSA-NP with SnCl2 and sodium gluconate and the stability of ^188Re -GCV-BSA-NP in vitro was measured. Results GCV-BSA-NPs was regular spheres with various size, and the diameter of most of them was in the range from 250 to 290 nm.The slower sustained release of GCV-BSA-NP was attain- able compared with the conventional dosage form of GCV ;The labeling yield was over 90%. The radiochemical purity of ^188Re-GCV-BSA-NP was 95% after purification. The specific activity was greater than 1.85×10^5 MBq/μmol. The labeled compound was stable at least for 24 h in PBS （pH 7.4） at 37℃. Conclusion This method of directly labeling raRe to BSA-NP with SnCl2 and sodium gluconate is stable and the high radiochemical purity was obtained.

关 键 词：^188RE 丙氧鸟苷 牛血清白蛋白 

分 类 号：R392.5[医药卫生—免疫学]