维生素A及其配体和TNF-α调节肠上皮细胞分泌片的表达  

Influence of VA and TNF-α on the Expression of Secretory Component in Caco-2 Cell

作　　者：刘冬妍[1] 丁鹏[1] 马力[1] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心,沈阳110004

出　　处：《中国医科大学学报》2009年第3期178-180,183,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30670947;30871158);辽宁省教委课题资助项目(05L466;2008783)

摘　　要：目的探讨维生素(VA)和肿瘤坏死因子α(TNF-α)对人结肠腺癌细胞细胞系(Caco-2)细胞表达分泌层(SC)的影响。方法采用免疫组化、ELISA、Westernblot、RT-PCR方法检测全反式维甲酸(ATRA)和TNF-α对Caco-2细胞分泌SC的影响。结果ATRA和TNF-α促进Caco-2细胞分泌SC,但在ATRA缺无时TNF-α作用较弱,当ATRA和TNF-α共作用时SC的产生明显增加,与无ATRA比较,用ATRA和TNF-α刺激后SC阳性细胞、培养上清液中游离SC、Caco-2细胞SC蛋白和SC基因的表达明显增加(P<0.01)。结论ATRA和TNF-α上调Caco-2细胞分泌SC,充分说明VA是维持黏膜完整性的重要因子。Objective To explore the influence of vitamin A （ VA ） and TNF-α on the expression of SC in Caco-2 cells. Methods The influence of VA and TNF-α on the expression of SC Caco-2 cells, a well-differentiated human epithelial cell line derived from a colonic carcinoma,was determined by immunocytochemistry,ELISA,Westem blot and Quantitative real-time PCR. Results TNF-α increased the expression of SC in Caco-2 cells, but this increase was notably lower. SC-positive eel/s, SC in culture supematants, plgR mRNA and protein expression of Caco-2 cells were upregulated when Caco-2 cells were treated with TARA and TNF-α, compared with those untreated with TARA （P 〈 0.01 ）. Conclusion ATRA interacting with TNF-α up regulates SC expression in Caco-2 cells, suggesting that VA is an important factor for maintaining mucosal integrity.

关 键 词：肿瘤坏死因子Α 维生素A 分泌片 CACO-2细胞 

分 类 号：R446.62[医药卫生—诊断学]