巢式聚合酶链反应检测不同人群隐匿性乙型肝炎病毒感染  

Determining the Infection Rate Of Occult Hepatitis B Virus in Various Kinds of People by Nested-Polymerase Chain Reaction

作　　者：杨朝国[1] 张玲英[2] 许建[3] 

机构地区：[1]成都中医药大学医学技术学院,四川成都610041 [2]四川省人民医院,四川成都610072 [3]成都市第九人民医院,四川成都610041

出　　处：《四川省卫生管理干部学院学报》2009年第1期6-7,18,共3页Journal of Sichuan Continuing Education College of Medical Sciences

基　　金：四川省卫生厅科研课题(编号060093)

摘　　要：目的:探讨不同人群隐匿性乙型肝炎病毒(HBV)感染情况。方法:采用巢式聚合酶链反应(PCR)检测268例健康献血员、386例健康体检自然人群、73例不明原因肝炎患者、26例丙型肝炎患者、38例肝硬化患者和36例肝癌患者血清HBV DNA。结果:隐匿性乙型肝炎病毒感染率分别为0.37%、4.92%、35.62%、19.23%、10.53%和16.67%。隐匿性HBV感染者中,抗HBc或抗HBc和抗HBe阳性者占51.29%。结论:隐匿性HBV感染在不同人群中广泛存在,其与抗HBc存在高度相关。Objective： To determine the infection rate of occult hepatitis B virus （HBV） in various kinds of people. Methods： About 700 cases of serum were determined by nested-polymerase chain reaction, which were respectively from 268 cases of blood donors, 386 cases of general healthy individuals, 73 cases of chronic cryptogenic hepatitis, 26 cases of chronic hepatitis C, 38 cases of cirrhosis, and 36 cases of hepatocellular carcinoma. Results： The infection rate of occult hepatitis B virus in these different kinds of people were 0.37%, 4.92%, 35.62%, 19.23%, 10.53% and 16.67% respectively.And there had 51.29% of cases being infected were positive for anti-HBc or for both anti-HBc and anti-HBe. Conclusion： Occult hepatitis B virus infection widely exists in various kinds of people, and it is highly related with anti-HBc.

关 键 词：隐匿性乙型肝炎病毒感染 巢式聚合酶链反应 乙型肝炎病毒核酸 

分 类 号：R446.6[医药卫生—诊断学]