白玉簪离体快速繁殖的研究  

Study on Rapid Propagation in vitro of Hosta plantaginea

作　　者：顾德峰[1] 王刚[2] 高晶芳[1] 赵和祥[1] 郭太君[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]吉林省白河林业局两江林场,白河133600

出　　处：《吉林农业大学学报》2009年第2期161-163,共3页Journal of Jilin Agricultural University

基　　金：吉林省科技发展重点项目(20060224)

摘　　要：以白玉簪（Hosta plantaginea Aschers）茎芽为外植体,MS为基本培养基,通过添加不同质量浓度的细胞分裂素（6-BA、ZT）和生长素（NAA）进行离体繁殖。结果表明：培养基中添加6-BA 2.0 mg/L＋NAA 0.2 mg/L不定芽增殖率最高,增殖倍数达到3.9,玉米素（ZT）对幼芽的增殖效果不如6-BA;NAA质量浓度0.05-1.0 mg/L都能诱导白玉簪生根,生根率达93%以上。A study was conducted on tissue culture and rapid propagation in vitro of the Hosta plantaginea, which took stem bud as explants and was euhured on the MS added with different concentration of the cytokinin （6-BA,ZT） and the auxin （NAA）. The results indicated that the most suitable culture condition of adventitious bud was 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L, rather than ZT, propagation multiple amounts to 3.9. Root can be induced within concentration of NAA 0.05-1.0mg/L, rooting rate was over 93 % .

关 键 词：白玉簪 茎芽 组织培养 

分 类 号：S682[农业科学—观赏园艺]