微生物源硝基还原酶催化反应的研究  

Primarily Research of the Chemical Reaction Catalyzed by Nitroreductase from Microorganism

作　　者：薛章荣[1,2] 徐文平[2,3] 旷文丰 陶黎明[2,3] 

机构地区：[1]华东理工大学上海市化学生物学(芳香杂环)重点实验室,上海200237 [2]上海市农药研究所,上海200032 [3]上海南方农药研究中心,上海201702

出　　处：《化学与生物工程》2009年第5期29-31,66,共4页Chemistry & Bioengineering

基　　金：上海市优秀学科带头人计划(B类)资助项目;上海市重点学科建设资助项目(B507)

摘　　要：在建立的微生物源硝基还原酶筛选模型上,从众多微生物菌株中筛选诱变到一株细菌134-13,其培养液能将对-(N-苯基甲酰基)邻硝基苯甲醚转化成对-(N-苯基甲酰基)邻氨基苯甲醚,在优化的培养基上培养22h,底物的转化率可达89.4%。Based on the self-made nitro-reductase screening model,a nitro-reductase producing strain 134-13 was obtained by screening and mutation from varieties of microorganisms. The broth of the strain could transfer the nitro group of substrate to the amino group when it was cultured on the optimized culture medium for 22 h,the conversion rate of substrate could reach 89.4%.

关 键 词：微生物转化 硝基还原酶 筛选模型 

分 类 号：O621.33[理学—有机化学] O621.254.2[理学—化学]