不同虫龄米缟螟所产三叶虫茶HPLC指纹图谱的研究  

HPLC fingerprint of Sanye Chongcha produced by different ages of Aglossa Dimidiata

作　　者：肖美凤[1] 文礼章[2] 许光明[1] 彭新君[1] 

机构地区：[1]湖南中医药大学药学院,中药学重点学科长沙410208 [2]湖南农业大学生物安全科学技术学院,长沙410128

出　　处：《中南药学》2009年第5期374-377,共4页Central South Pharmacy

基　　金：湖南省卫生厅资助项目(206080)

摘　　要：目的建立不同虫龄米缟螟幼虫所产三叶虫茶的HPLC指纹图谱。方法采用HPLC法,色谱条件:Sino-chrom ODS-BP柱(250 mm×4.6 mm,5μm);流动相:乙腈-1%醋酸水溶液,梯度洗脱;流速为1.0 mL.min-1;检测波长为285 nm;柱温为40℃。结果通过检测6批次三叶虫茶,建立其HPLC指纹图谱,共标定了26个共有指纹峰,并且比较了其中的白杨素苷和白杨素的含量。结论所用方法稳定、重复性好,可用于三叶虫茶的质量控制。Objective To develop an HPLC fingerprint of Sanye Chongcha produced by different ages of Aglossa Dimidiata. Methods HPLC was used. Chromatogram column was Sinoehrom ODS-BP （250mm×4.6mm, 5 btm）; the mobile phase consisted of acetonitrile-water containing 1% acetic acid with gradient elution; the flow rate was 1.0 mL· min^-1. The detection wavelengh was 285 nm and column temperature was 40℃. Results After detecting 6 different batches of Sanye Chongcha, an HPLC fingerprint of Chongeha was developed with 26 common peaks simultaneously and the content of chrysin-glucoside and chrysin was compared. Conclusion The method is stable and repeatable, which can be used for the quality control of Sanye Chongcha.

关 键 词：三叶虫茶 白杨素苷 白杨素 指纹图谱 HPLC法 

分 类 号：R284[医药卫生—中药学] O234[医药卫生—中医学]