鸡产气荚膜梭菌遗传多样性的REP-PCR分析  

Analysis of genetic diversity of Clostridium perfringens from chicken by REP-PCR

作　　者：郑晓丽[1,2] 倪学勤[1,3] 曾东[1,3] JOSHUA Gong 宋振银[1,2] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,四川雅安625014 [2]四川农业大学资源环境学院,四川雅安625014 [3]动物疫病与人类健康四川省重点实验室,四川雅安625014 [4]Food Research Program,Agriculture and Agri-Food Canada,Guelph

出　　处：《中国兽医科学》2009年第5期383-388,共6页Chinese Veterinary Science

基　　金：四川省科技厅国际合作项目(HH20080016)

摘　　要：为探讨鸡产气荚膜梭菌流行现状的调查方法,采用REP-PCR(repetitive extragenic palindromicPCR)技术对四川省10个规模化鸡场分离得到的34株A型产气荚膜梭菌进行了遗传多样性分析,并与AFLP(amplified fragment length polymorphism)方法进行了分型比较。结果显示,尽管REP-PCR分型方法只能将34株产气荚膜梭菌分为7个亚型,但是,仍然表现出对产气荚膜梭菌菌株的较高的鉴别能力,不失为一种简便、快速、可靠的产气荚膜梭菌分型方法。经对亚型分布情况进行分析,表明产气荚膜梭菌的流行特点与地区差异相关,不同鸡场产气荚膜梭菌的亚型差异明显,而同一鸡场的亚型较单一,以一种亚型为主,交叉有少数其他亚型;优势基因型为REP-PCR基因1型、2型和3型。证实,REP-PCR技术适用于鸡产气荚膜梭菌遗传多样性分析。To validate an investigation method for the epidemiology of Clostridium perfringens in chicken,genetic diversities of 34 strains of C. perfringens type A isolated from healthy chicken of 10 different integrated chicken farms in Sichuan Province were analyzed by repetitive extragenic palindromic PCR （REP-PCR） and amplified fragment length polymorphism（AFLP） methods. The different results were compared between the two methods. The 34 strains were differentiated into 7 subtypes by REP-PCR,which was a simple, fast and reliable method for subtyping of C. perfringens. The results revealed that the genetic types were correlated closely with the origins of the isolates. The difference of genetic types was significant among different chicken farms. A subtype of C. perfringens was main on any one chicken farm with a few other subtypes. The dominant genotypes were REP-PCR genotypes 1,2,and 3. These results demonstrated that REP-PCR was suitable for subtyping of C. perfringens isolates.

关 键 词：产气荚膜梭菌 遗传多样性 基因组重复序列聚合酶链式反应 鸡 

分 类 号：S852.616.3[农业科学—基础兽医学] Q503[农业科学—兽医学]