刚地弓形虫代谢分泌抗原对山羊IFN-γ、TNF-α、IL-2和IL-4表达水平的影响  

Effects of Toxoplasma gondii excreted/secreted antigens on the expression level of IFN-γ,TNF-α,IL-2 and IL-4 in goat

作　　者：芦赟[1,2] 张燕丽[1,2] 曹丽艳[1,2] 王艳华[2] 苟惠天[1,2] 付宝权[2] 李文卉[2] 张德林[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2009年第5期395-400,共6页Chinese Veterinary Science

基　　金：国家“十一五”科技支撑计划项目(2007BAD40B05);公益性行业(农业)科研专项经费项目(200803017);甘肃省重大专项科技项目(2GS063-A43-013)

摘　　要：为了探索刚地弓形虫代谢分泌抗原(E/SA)对山羊的免疫保护机制,将山羊随机分为6组,即E/SA组、E/SA+CpG组(未乳化)、E/SA+CpG组、E/SA+IL-2组、E/SA+IL-2+CpG组、对照组,每组3只,分别于免疫前、免疫后第2周、免疫后第4周、感染后第1周及感染后第2周采集山羊血清,用ELISA法检测IFN-γ、TNF-α、IL-2、IL-4的表达水平。结果显示,各免疫组免疫后IFN-γ、TNF-α、IL-2、IL-4的含量分别为175.40～215.60、89.95～253.80、230.40～301.50、39.20～54.20pg/mL,而免疫前分别为14.00～18.75、30.91～42.20、10.75～18.30、20.40～24.60pg/mL,对照组分别为16.50～36.56、37.75～58.20、11.50～21.75、21.61～27.60pg/mL,免疫组在免疫后体内IFN-γ、TNF-α、IL-2、IL-4的含量明显升高,均显著高于对照组(P<0.05)。表明,E/SA可引起IFN-γ、TNF-α、IL-2、IL-4水平的升高,说明E/SA免疫后可引起山羊较强的细胞免疫和体液免疫应答。To elucidate the effect of Toxoplasma gondii excreted/secreted antigens on goat＇s immune function,goats were randomly divided into 5 immune groups of E/SA, E/SA＋CpG （without emulsification）,E/SA＋CpG,E/SA＋IL-2,E/SA＋IL-2＋CpG,and the control group with 3 each. Sera were collected and the level of IFN-γ,TNF-α,IL-2 and IL-4 was analyzed by ELISA before immunization,on week 2 post-immunization,on week 4 post-immunization, on week 1 post-infection and on week 2 post-infection, respectively. The levels of IFN-γ, TNF-α, IL-2 and IL-4 were 175.40 - 215.60,89.95 - 253.80,230.40 - 301.50 and 39.20-54.20 pg/mL after immunization,respectively,but were 14.00-18.75,30.91-42.20, 10. 75-18.30,20.40-24.60 pg/mL pre-immunization in the 5 immune groups. The levels were 16.50- 36.56,37.75-58.20,11.50-21. 75 and 21.61-27.60 pg/mL in the control group. The levels of IFN-γ, TNF-α,IL-2,IL-4 in the 5 immune groups were significantly higher than that in the control group（P〈0.05） post-immunization. The results that E/SA elevated the levels of IFN-γ,TNF-α,IL-2 and IL-4 showed that E/SA could result quickly in cellular immune response and humoral immune response.

关 键 词：刚地弓形虫 代谢分泌抗原 干扰素-Γ 肿瘤坏死因子-α 白细胞介素-2 白细胞介素-4 

分 类 号：S852.723[农业科学—基础兽医学]