chTERT mRNA表达及端粒酶活性在氯化镧诱导MDCC-MSB1细胞凋亡中的作用  

Roles of the chTERT mRNA expression and telomerase activity in apoptosis of MDCC-MSB1 cells induced by LaCl_3 in vitro

作　　者：张子威[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2009年第5期437-444,共8页Chinese Veterinary Science

基　　金：黑龙江省研究生创新科研资金项目(YJSCX2008-097HLJ)

摘　　要：为探讨chTERT mRNA表达及端粒酶活性在LaCl3诱导MDCC-MSB1细胞凋亡中的作用。将肿瘤细胞常规培养于RPMI1640培养液中,加入终浓度为3mmol/L的LaCl3共培养,分别于培养第12、24、36和48h,用透射电镜观察LaCl3致MDCC-MSB1细胞的形态学变化,应用流式细胞仪检测细胞凋亡,以TRAP-PCR银染法检测端粒酶活性,以实时荧光定量PCR法检测chTERT基因mRNA的表达量变化。结果显示,3mmol/L的LaCl3作用第12、24、36和48h,透射电镜和流式细胞仪法均检测到明显的细胞凋亡变化,端粒酶活性下降,chTERT mRNA的表达量下降,并呈时间-效应关系。证实,LaCl3可通过降低chTERT mRNA的表达量抑制MDCC-MSB1细胞的端粒酶活性而诱导其发生凋亡。The tumor cell MDCC-MSB1 was cultivated regularly in RPMI 1640 solution with LaCl3 （final concentration： 3 mmol/L）. At hour 12,24,36, and 48 post-cultivation, the morphology changes were observed by transmission electron microscope. The apoptosis were identified by flow cytometry,the telomerase activity was examined by TRAP-PCR silver staining and the expression quantity alteration of chTERT mRNA was examined by real-time fluorescence quantitative PCR. According to transmission electron microscope and flow cytometry,obvious apoptosis was observed at four time points. The telomerase activity and expression quantity of chTERT mRNA decreased along with prolongation of time. The results indicated that LaCl3 would be able to induce MDCC MSB1 apoptosis by way of reducing of chTERT mRNA expression and inhibiting telomerase activity.

关 键 词：氯化镧 MDCC-MSB1 细胞凋亡 端粒酶活性 端粒酶逆转录酶MRNA 

分 类 号：Q279[生物学—细胞生物学]