肌成纤维细胞与医源性胆管狭窄的相关性研究  

The pertinency research of myofibroblast and iatrogenic biliary stricture

作　　者：马晓[1] 赵国忠[1] 

机构地区：[1]宁夏医科大学附属医院肝胆外科,银川市750001

出　　处：《临床合理用药杂志》2009年第8期7-8,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的探讨肌成纤维细胞(MFB)在医源性胆管狭窄形成过程中的作用及医源性胆管狭窄的形成机制。方法通过建立家兔肝外胆管损伤修复模型,分别于术后1周、3周、3个月、6个月取材行光镜、电镜观察,并免疫组织化学染色观察α-平滑肌肌动蛋白(α-SMA)。结果MFB功能活跃,持续存在于整个胆道愈合过程;α-SMA表达于肌成纤维细胞胞浆,术后1周～6个月表达均较强,与正常对照比较差异有统计学意义(P<0.01),术后各期表达差异无统计学意义(P>0.05)。结论MFB功能活跃,持续存在,是导致胆道瘢痕性挛缩和管腔狭窄的主要原因。Objective To study the function of myofibroblast in the process of iatrogenic biliary stricture;To study the mechanism of iatrogenie biliary stricture. Methods A model of traumα -repair of extrahepatie bile duct in the rabbit was made,The anastomosis tissue on the 1 st week,3rd week, 3rd month,6rd month after operation was observed under microscope and transmission electron microscopy and immunohisisto chemical SP staining of α-SMA examined. Results Myofibrohlasts functioned actively and lasted for the whole process,α--SMA staining was observed at the myofibrohlasts ,expressed strong from the 1 stweek to 6thmonth after operation （P 〈 0.01 ）. There was no significant difference among the experimentalgroups （P 〉 0. 05）. Conclusion Myofibroblast functions actively and lasts a longer time, which was the main cause for scar contracture and stricture of bile duct.

关 键 词：肌成纤维细胞 胆管狭窄 医源性 

分 类 号：R657.4[医药卫生—外科学]