纹缟虾虎鱼精子的主要生物学特性  

Studies on main biological characteristics of Tridentiger trigonocephalus sperm

作　　者：黄晓荣[1,2] 庄平[1,2,3] 章龙珍[1,2,3] 闫文罡[1,2] 江琪[1,2] 陈丽慧[1,3] 冯广朋[1,2] 张涛[1] 赵峰[1,2] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海海洋大学水产与生命学院,上海201306 [3]大连水产学院生命科学与技术学院,辽宁大连116023

出　　处：《上海海洋大学学报》2009年第3期275-281,共7页Journal of Shanghai Ocean University

基　　金：国家科技基础条件平台基金(2006DKA30470-004);国家自然科学基金(NSFC30490230WP4-003);中央级公益性科研院所基本科研业务费专项资金项目(2007T02);上海市高校水产养殖学E-研究院建设项目(E03009);上海市长江口中华鲟自然保护区科研专项项目

摘　　要：选取健康成熟的纹缟虾虎鱼(Tridentiger trigonocephalus)进行人工采精,设定不同的盐度和温度梯度,观察其对精子活力及寿命的影响。测定了精子的大小、密度,并观察了精子的超微结构。结果表明,随着盐度和温度的上升,精子的活力和寿命都先增加后降低,在盐度5和温度19℃时,精子的活力和寿命分别达到最高。测得精子密度为(1.73×109±0.08×109)/mL,细胞核长径为(0.98±0.12)μm,短径为(0.81±0.08)μm,鞭毛的长度为(7.28±1.06)μm。电镜结果表明:纹缟虾虎鱼精子由头部和尾部(鞭毛)二部分组成,头部的细胞核近圆形,内有核空泡。精子细胞质膜与核膜间有较大间隙,两者之间分布有细胞质和线粒体。中心粒复合体位于植入窝中,由近端中心粒和基体两部分组成。袖套位于核后端,呈一筒状,两侧不对称,袖套腔较为狭窄,两侧分布有线粒体和少量囊泡。鞭毛的核心结构是轴丝,轴丝为典型的"9+2"结构,从轴丝的横切面观察到轴丝外面的质膜呈不规则分布。Healthy male Tridentiger trigonocephalus were selected and their sperm were collected. The effects of salinity and temperature on the motility and longevity of spermatozoa were studied. The density and size of the sperm were also measured, and the ultrastructure of the spermatozoa was observed. The results showed： when salinity and temperature rose, the spermatozoa motility and longevity first increased then dropped. At the salinity 5 and at the temperature 19 ℃, the sperm had the best motility and longest life respectively. Average density of the spermatozoa was 1.73 × 10^9 ±0.08 × 10^9/mL. Long diameter of karyon was （0.98 ±0.12） μm and short diameter was （0.81 ± 0.08 ） μm. Flagellum length was （7.28 ± 1.06） μm. Uhrastructure showed that the sperm of Tridentiger trigonocephalus consisted of two parts ： head and flagellum. The spermic head was almost round in shape. Within condensed chromatin there are some less dense areas called nuclear vacuoles. The space between the membrane of the nucleus and cytoplasm was relatively large, in the space there were some cytoplasm and mitochondria. The centriolar complex was inside implantation fossa and consisted of proximal centriole and basal body. The proximal centriole and basal body were located on the upside and underside of the implantation fossa respectively. The sleeve was located in the back-end of the nucleus, and the two wings of the sleeve were asymmetry. The central space of sleeve was incommodious, and there were some mitochondria and vesicle on the two sides. The central structure of the tail was axoneme, and the axoneme formed a typical 9 ＋ 2 pattern. A cross section of the axoneme showed the cytoplasm membrane outside the axoneme was irregular

关 键 词：纹缟虾虎鱼 精子活力 超微结构 中心粒复合体 “9+2”微管结构 

分 类 号：S917[农业科学—水产科学]