不同浓度甘油和pH对DEAE-纤维素柱分离伊氏锥虫效果的影响  

THE EFFECT OF GLYCEROL CONCENTRATIONS IN DIFFERENT pH VALUE ON THE PURIFICATION OF TRYPANOSOMA EVANSI USING DEAE 52 COLUMN CHORMATOGRAPHY

作　　者：蔡建平[1] 沈永林[1] 潘红杰[1] 汪志楷[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《畜牧与兽医》1998年第3期99-101,共3页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金

摘　　要：在不同ｐＨ条件下以不同浓度甘油破坏鼠红细胞后，用ＤＥＡＥ－５２纤维素柱层析分离纯化伊氏锥虫（Ｔｒｙｐａｎｏｓｏｍａｅｖａｎｓｉ），结果表明在ｐＨ７．４时，２０％甘油能溶解大约９５％的大、小鼠红细胞；经此处理后，对Ｔ．ｅｖａｎｓｉ的回收率和活力无不良影响，可明显提高其分离纯化效果。作者还观察了渗透压和ｐＨ条件改变对Ｔ．ｅｖａｎｓｉ的影响。Trypanosoma evansi in the rat or mice blood were purified using DEAE 52 Column Chromatography after the lysis of red blood cells(RBC)with Glycerol in different concentrations under different pH Value.It was shown that about 95% RBC were lysed in 20% Glycerol of pH7.4,and that the recoverable rate and activity of T.evansi were not affected.The resistance of T.evansi to the changes of osmosity and pH value were also investigated.

关 键 词：伊氏锥虫 甘油 DEAE-52 纤维素柱层析 

分 类 号：S852.7[农业科学—基础兽医学]