PDF1.2基因在转草酸氧化酶基因甘蓝型油菜中的表达  

Expression of PDF1.2 in Oxalate Oxidase Transgenic Oilseed Rape (Brassica napus L.)

作　　者：冀瑞琴[1,2] 董祥柏[2] 冯辉[1] MARTIN Barbetti 高荣村[2] 刘月英[2] 刘胜毅[2] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161 [2]中国农业科学院油料作物研究所,武汉430062 [3]School of Plant Biology,Faculty of Natural and Agricultural Sciences,the University of WesternAustralia,Crawley,WA,60099,Australia

出　　处：《植物生理学通讯》2009年第5期479-482,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30671344、30800748);中国博士后科学基金(20070411080)

摘　　要：采用RT-PCR方法检测茉莉酸(JA)/乙烯(ET)依赖性信号途径中关键基因PDF1.2在转草酸氧化酶基因(OXO)油菜株系与未转化对照中的表达差异。结果表明,在转基因油菜不同株系中PDF1.2都有不同程度的上调表达,预示着转OXO油菜对菌核病的抗性增强可能与激活JA/ET依赖性信号途径有关。The expressions of PDF1.2, a key gene in the jasmonic acid （JA）/ethylene （ET）-dependent signaling pathway, were measured in oxalate oxidase （OXO） transgenic and non-transgenic oilseed rapes （Brassica napus） by RT-PCR. The results showed that the expression of PDF1.2 was up-regulated in different OXO transgenic oilseed rape lines, which indicated that the mechanism of disease resistance to Sclerotinia sclerotiorum was related to the induction of the JA/ET-dependent signaling pathway.

关 键 词：甘蓝型油菜 菌核病 PDF1.2 转草酸氧化酶基因 

分 类 号：S565.4[农业科学—作物学]