寒冷刺激对部分睡眠剥夺小鼠血细胞参数的影响  

EFFECTS OF COLD STRESS ON BLOOD CELL PARAMETERS OF PARTIAL SLEEP DEPRIVATION MICE

作　　者：成良[1] 王娜[1] 刘萍[1] 廖晓梅[1] 陈其才[1] 

机构地区：[1]华中师范大学生命科学学院神经生物学实验室,遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《中国应用生理学杂志》2009年第2期156-160,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30700208,30670695);湖北省自然科学基金资助课题(2007ABA150)

摘　　要：目的:探讨寒冷刺激,部分睡眠剥夺,以及部分睡眠剥夺的基础上再给予寒冷刺激等处理对小鼠血细胞参数的影响。方法:昆明小鼠24只,随机均分为4组(n=6):对照组、寒冷组、不完全睡眠剥夺组和不完全睡眠剥夺加寒冷组。寒冷组每天给予(10±2)℃的低温处理4 h,不完全睡眠剥夺组每天18∶00至次日9∶00剥夺睡眠,不完全睡眠剥夺加寒冷组在每天睡眠剥夺的基础上再给予4 h寒冷刺激。连续处理4 d后采血检测血常规和血沉率。结果:与对照组相比,寒冷刺激可使小鼠血液中淋巴细胞含量(P<0.05)以及百分比(P<0.01)显著增加;部分睡眠剥夺可使小鼠血液白细胞和淋巴细胞含量(P<0.05)及百分比(P<0.01)明显降低。不完全睡眠剥夺加寒冷刺激处理后与其它三组相比小鼠血液白细胞和淋巴细胞含量显著降低(P<0.01),而血沉率则显著升高(P<0.01)。结论:部分睡眠剥夺会抑制机体免疫能力,在部分剥夺睡眠的基础上再给予寒冷刺激则将进一步抑制机体免疫能力并使血沉率加快,降低机体对外界环境变化的适应能力。Aim： To investigate the effect of cold, partial sleep deprivation, partial sleep deprivation plus cold on blood routine parameters and erythrocyte sedimentation rate of mice. Methods： Twenty-four Mus musculus mice were divided into four groups （ n = ① randomly： Ocontrol, ②cold group： mice were treated with （10± 2）℃ cold stimulation for four hours per day, ③partial sleep deprivation group： mice were deprived sleep from 18：00 to 9：00 next day, ④partial sleep deprivation plus cold group： mice were treated with cold stimulation based on partial sleep deprivation. After four days treatment, the mice were sacrificed and the blood was collected to detect the blood routine parameters and erythrocyte sedimentation rate. Results： Compared with the control, cold stimulation would increase the contents and proportion of lymphocyte significantly. Partial sleep deprivation would decrease the white blood cell contents, lymphocyte contents and lymphocyte proportion significantly. After treated with cold stimulation plus partial sleep deprivation, the white blood cell and lymphocyte contents decreased and the erythrocyte sedimentation rate increased evidently compared with other three groups. Conclusion： Partial sleep deprivation could inhibit immune function of the mice. When the mice were treated with cold stimulation plus partial sleep deprivation, the immune function of the mice would be inhibited further more and at the same time the erythrocyte sedimentation rate increased significantly.

关 键 词：寒冷 部分睡眠剥夺 血常规 血沉率 

分 类 号：Q461[生物学—生理学]