谷氨酰胺对溃疡性结肠炎小鼠结肠损伤的影响  

EFFECTS OF GLUTAMINE ON THE COLON OF MICE SUBJECTED TO COLITIS GRAVIS

作　　者：李金敏[1] 贾海燕[1] 王俊杰[1] 于倩[1] 李术[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国应用生理学杂志》2009年第2期268-272,共5页Chinese Journal of Applied Physiology

摘　　要：目的:探讨谷氨酰胺(Gln)对溃疡性结肠炎小鼠结肠黏膜损伤的影响。方法:将64只35日龄中国昆明鼠随机分为4组(n=16):即Ⅰ组(空白对照组)、Ⅱ组(模型组)、Ⅲ组(Gln低剂量组)、Ⅳ组(Gln高剂量组),Ⅱ、Ⅲ、Ⅳ组采用乙酸灌肠法人工复制小鼠溃疡性结肠炎模型,模型复制成功后Ⅰ、Ⅱ组均给予一定量的生理盐水灌服,Ⅲ、Ⅳ组分别灌服与生理盐水量相等的低(2 mmo.lkg-1bw)、高(4 mmol.kg-1bw)两种浓度的Gln溶液,连续灌服7 d后将小鼠处死。观察结肠病理组织学变化,检测血清内毒素含量,结肠组织的抗氧化水平、髓过氧化物酶(MPO)活性。结果:Gln减轻了由结肠炎造成的结肠黏膜的病理损伤,一定程度上缓解了结肠炎时血清内毒素急剧升高、结肠抗氧化水平降低及髓过氧化物酶活性升高等现象。结论:Gln对溃疡性结肠炎所致小鼠结肠损伤具有一定的修复作用。To investigate the effects of glutamine on the colonic mucosa of mice subjected to colitis gravis. Methods： 64 Kunming mice were divided randomly into 4 groups（ n = 16） ： healthy group： animals not subjected to colitis; model group： animals subjected to colitis gravis but without glutamine supplementation; low-Gin group： animals subjected to colitis gravis and with low dose of glutamine supplementation; high- Gln group： animals subjected to colitis gravis and with high dose of glutamine supplementation. Animals belonging to the control, the low-Gln, the high-Gln groups were subjected to coloclysis by HAC to be colitis gravis animals. When the models were established, the healthy and the control groups were given some isotonic Na chloride by intragastric administration. The low-Gln group and the high-Gln group were given the same volume but different concentration of glutamine（low-Gln group-2 mmol· Kg^-1 bw, high-Gin group-2 mmol· Kg^-1 bw） for 7 days. Then the mice were sacrificed, the pathohistological changes of the colon were observed, besides, the content of endotoxin in the blood serum, the level of counteracting oxidation and the activities of MPO of the colon tissue were determined. Results： The glutamine lessened the pathological injures in the colon and relieved the step up of the content of endotoxin in the blood serum , the step down level of counteracting oxidation and the step up activity of MPO in the colon tissue, which were caused by colitis gravis. Conclusion： The glutamine can protect the colon of mice subjected to colitis gravis.

关 键 词：谷氨酰胺 结肠炎 小鼠 

分 类 号：R333[医药卫生—人体生理学]