大孔吸附树脂纯化丹参水溶性总酚酸工艺研究  

Purification of Total Salvianolic Acid Using Macroporous Adsorption Resin

作　　者：李丹[1,2] 宋洪涛[1] 初阳[3] 陈大为[4] 刘丹[4] 鄢璐[4] 

机构地区：[1]南京军区福州总医院药学科,福州市350025 [2]沈阳医学院奉天医院药剂科,沈阳市110024 [3]中国医科大学附属第一医院药剂科,沈阳市110024 [4]沈阳药科大学药学院,沈阳市110016

出　　处：《中国药房》2009年第15期1142-1145,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(30200363)

摘　　要：目的:研究大孔吸附树脂纯化丹参水溶性总酚酸的工艺。方法:以丹酚酸B、丹参素及原儿茶醛的转移率及在固形物中的含量作为指标,考察乙醇浓度、树脂药材比、树脂柱径高比、洗脱液流速等工艺条件,并与乙醇沉淀法进行纯化效果的比较。结果:纯化工艺为采用HPD300型大孔吸附树脂,15%和50%乙醇为梯度洗脱液,树脂药材比为5∶1,树脂柱径高比为1∶7,洗脱液流速为2.5BV.h-1。在该条件下,丹酚酸B、丹参素及原儿茶醛的转移率分别为81.8%、95.3%及97.5%,在固形物中的含量分别为24.70%、4.60%和0.41%,合计达到29.71%。,大孔吸附树脂法的各指标成分转移率与乙醇沉淀法相当,而各指标成分在固形物中的含量约为乙醇沉淀法的2.8倍。结论:大孔吸附树脂法对丹参水提液的纯化效果优于传统的水提醇沉法,且操作容易,节省溶剂,值得在生产中推广使用。OBJECTIVE： To study the purification conditions of total salvianolic acid using macroporous adsorption resin. METHODS： The transfer rates of salvianolic acid B, danshensu and protocatechuic aldehyde and their contents in the solid substance were taken as indexes to investigate the purification conditions including the alcohol concentration, the ratio of resin to material drug, the ratio of diameter to height of resin column and the flow speed of elution etc. The purification efficacy of total salvianolic acid by macroporos resin was compared with that by alcohol sedimentation. RESULTS： The optimum purifi- cation conditions were as follows： HPD300 macroporos resin was used using 15% or 50% alcohol as gradient elution agent with the ratio of resin to material drug at 5 ： 1, the ratio of diameter to height of resin column at 1 ： 7 and the flow speed of elution at 2.5 BV · h^-1.Under the above purification conditions, the transfer rate of salvianolic acid B, danshensu and protocatechuic were 81.8%, 95.3% and 97.5%, respectively, and their contents in solid substance were 24.7%, 4.6% and 0.41%, respectively, totaling 29.71% Ifor the three components. The macroporos resin method was equivalent to alcohol sedimentation in transfer rates of salvianolic acid B, danshensu and protocateehuic aldehyde, but the contents of the components in the solid substance by macroporos resin method was about 2.8 times as compared by alcohol sedimentation. CONCLUSION： The macroporos resin- method is superior to water extraction and alcohol sedimentation in purification efficacy of total salvianolic acid in that it is easy to operate and solvent - saving thus deserving to be popularized in production.

关 键 词：大孔吸附树脂 丹参总酚酸 纯化 

分 类 号：R284.2[医药卫生—中药学] R283[医药卫生—中医学]