川芎嗪对免疫介导再生障碍性贫血小鼠骨髓细胞CD_(34)抗原表达的影响  

Effect of Ligustrazine on CD_(34) Antigen Expression of Bone Marrow Cells in Immune-Mediated Aplastic Anemia Mice

作　　者：舒砚君[1] 孙汉英[1] 董凌莉[1] 徐慧珍[1] 路武[1] 刘文励[1] 

机构地区：[1]同济医科大学附属同济医院,武汉430030

出　　处：《中国中西医结合杂志》1998年第2期107-108,共2页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金!39470883;湖北省自然科学基金!96J080

摘　　要：探讨川芎嗪对免疫再生障碍性贫血（再障）小鼠骨髓细胞ＣＤ_（３４）抗原分子表达的影响。方法：建立免疫介导的再障小鼠模型， ６．０Ｇｙ～^（６０）Ｃｏγ－射线照射和尾静脉输注淋巴细胞造成小鼠再障。分为正常组、再障组（对照组）、川芎嗪组，除正常组外均胃饲川芎嗪注射液 ４ｍｇ／次，１天 ２次，第 １０天用流式细胞仪检测各组小鼠骨髓细胞膜上 ＣＤ_（３４）抗原表达量。结果：川芎嗪 ＣＤ_（３４）抗原表达量的荧光强度（７７．６±６．５）明显高于再障组（６８．６±４．５，Ｐ＜０．０５），与正常组（８０．０±２．６）比较无明显差异。结论：川芎嗪通过影响骨髓微环境促进免疫再障小鼠的造血干、祖细胞增生，增加ＣＤ_（３４）抗原分子的表达。To explore the effect of ligustrazine on CD_(34) antigen expression of bone marrow cells in imrnunemediated aplastic anemia (AA)mice. Methods: The model of immune aplastic anemia mice was induced by means of 6.0Gy^(60)Co γ-ray irradiation and lymphocyte infusion through tail vein. The mice were divided into 3 groups: the normal group, the AA control group and the ligustrazine group. Mice of the ligustrazine group were fed by 4 mg of ligustrazine injection twice a day by gastrogavage. On the 10th day, CD_(34) antigen expression intensity of bone marrow cell membrane was measured by flow cytometer analysis system. Results: CD_(34) antigen expression intensity of ligustrazine group was 77.6±6. 5, with no statistic difference from that in the normal group (80.0±2. 6), while that of the control group was much higher (68.6±4.5, P<0.05). Conclusions: Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through influencing on bone marrow micro-environment so as to increase the CD_(34) antigen expression of bone marrow cells.

关 键 词：川芎嗪 再生障碍性贫血 CD34抗原 

分 类 号：R259.565[医药卫生—中西医结合]