美伐他汀产生菌橘青霉原生质体的制备与再生  

Protoplast Forming and Regenerating from Mevastatin-Producing Fungus Penicillium citrinum

作　　者：张文琦[1] 胡昌华[1] 

机构地区：[1]西南大学药学院,现代生物医药研究所,重庆400716

出　　处：《西南大学学报（自然科学版）》2009年第5期117-120,共4页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科技攻关项目(2003-8119);西南师范大学高新技术培育项目(XSGX0605)

摘　　要：确定美伐他汀产生菌橘青霉(Penicillium citrinum)的原生质体的制备及再生的最佳条件.通过正交实验设计的方法,确定了接种量约108个孢子时,于85 mL YPL-20液体培养基中25℃,210 rpm,培养60 h,0.5%纤维素酶和蜗牛酶等比例混合酶液,在30℃酶解2 h,以蔗糖为高渗稳定剂的PGA培养基,原生质体再生形成率可达42.45%.本结果为深入研究橘青霉菌株的原生质体诱变、构建基因工程菌和提高美伐他汀产量奠定基础.This study was aimed at exploring an optimal condition for the process of formation and regeneration of protoplasts from the mevastatin-producing fungus Penicilliurn citrinum. The results of an orthogonal design experiment identified that the maximum formation rate （42.45 %） was achieved when approximately 10^8 spores were incubated in 85ml YPL-20 liquid medium for 60 hours at 25℃, and then a 0. 5% mixture of cellulose and snailase was added and digested at 30℃ for 2 hours in sucrose-PGA medium. This work has laid a foundation for the further research on protoplast mutagenesis, genetic engineering strain construction and output increase of mevastatin.

关 键 词：橘青霉 原生质体 正交设计 形成率 

分 类 号：R972[医药卫生—药品]