抗性早熟颗粒中药对性早熟SD大鼠模型雌二醇的调节及相关研究  

Adjustment of Anti-proeotia Keli on Aquadiol of SD Rat Model with Sexual Precocity

作　　者：陈祺[1] 戴方伟[2,3] 邵国民[1] 萨晓婴[2] 

机构地区：[1]浙江中医药大学第一附属医院儿科,浙江省杭州市邮电路54号310006 [2]浙江省医学科学院实验动物中心 [3]浙江大学动物科学学院

出　　处：《世界中医药》2009年第3期164-166,共3页World Chinese Medicine

摘　　要：目的:研究抗性早熟颗粒中药对特发性性早熟模型大鼠的治疗作用。方法:将出生4天内SD大鼠分为正常组、模型组、药物组。模型组、药物组皮下注射达那唑300μg,正常组未做处理。药物组在15日龄时开始灌喂抗性早熟颗粒药,正常组给予等量的自来水。15日后每日观察阴道开口并进行阴道涂片,于性周期稳定后的第1个发情前期时称体重、采样(血清,子宫,卵巢),用放射免疫法观察SD大鼠血清E2,FSH和LH的分泌,采用子宫和卵巢的湿重/采样体重求得脏器系数。结果:和正常组比较,模型组SD大鼠的阴门开启和建立性周期的时间显著提前(P<0.05),药物组SD大鼠的阴门开启和建立性周期时间延缓;卵巢脏器系数差异显著(P<0.05),模型组最高,药物组较之明显缩小,但药物组的卵巢脏器系数与正常组无明显差异;模型组E2最高,药物组和正常组低,且控制效果与药物浓度呈正比。结论:300μg达那唑皮下注射5～8日龄SD大鼠能诱导性早熟模型。抗性早熟颗粒药对性早熟模型SD大鼠有治疗作用,能延迟SD大鼠阴道开口平均日龄,降低卵巢脏器系数,控制E2过度升高,延缓性发育,为抗性早熟颗粒中药临床治疗性早熟提供科学实验依据。Objective：To research the effect of Anti-proeotia Keli on SD rat model of idiopathic sexual precocity. Methods：Within 4 days of birth, rats were grouped, on D5 -8 received H. injection of Danazol （300μg） except for normal group, from D15 were given keli or water aa, 15 days later, were observed vagina opening, made VS, weighed, took serum, uterus, and ovaries samples before 1st empathema, determined E2, FSH and LH levels by RI, calculated uterus/ovaries ratios （WW/BM）. Results：①vagina opening and sex cycle beginning ： by comparison with the normal group, the model advanced in time （ P 〈 0. 05 ） and the medicine group was delayed ; ②ovaries ratio： model group had the highest ratio （ P 〈 0.05 ） where keli group was statistically the same with the normal group; ③E2 level： model group was higher than the other two groups and effect positively correlated with concentration. Condusion：H. injection of Danazol （300μg） can produce sexual precocity in SD rats of 5 - 8 day of birth. Anti-proeotia Keli is effective for adjusting sex development by delaying vagina opening,lowering ovary ratio and E2 level.

关 键 词：性早熟/中医药疗法 抗性早熟颗粒中药 雌二醇 

分 类 号：R259.862.3[医药卫生—中西医结合] R-33[医药卫生—中医内科学]