镉、镍对小鼠肢芽细胞增殖和分化的影响  

Effects of cadmium and nickel on the proliferation and differentiation of mouse embryo limb bud cell

作　　者：罗扬[1] 周树森[1] 赵树芬[1] 

机构地区：[1]北京医科大学劳动卫生教研室

出　　处：《中华劳动卫生职业病杂志》1998年第1期15-18,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家教委博士点基金

摘　　要：目的探讨镉、镍单独及联合作用的发育毒性特点。方法应用小鼠胚胎肢芽细胞微团培养法观察ＣｄＣｌ２、ＮｉＣｌ２单独及联合染毒对胚胎肢芽细胞增殖和分化的影响。结果０．１～１．２μｇ／ｍｌＣｄＣｌ２或５．５～２７．５μｇ／ｍｌＮｉＣｌ２对胚胎肢芽细胞的增殖均有明显的抑制作用；０．４～１．２μｇ／ｍｌＣｄＣｌ２或５．５～２７．５μｇ／ｍｌＮｉＣｌ２也可明显抑制胚胎肢芽细胞的分化，但ＣｄＣｌ２在０．１和０．２μｇ／ｍｌ时可促进细胞分化。ＣｄＣｌ２和ＮｉＣｌ２联合染毒对胚胎肢芽细胞的增殖和分化的抑制分别表现为相加和加强作用。结论Ｃｄ和Ｎｉ可能通过某种共同机制而对胚胎肢芽细胞的增殖和分化的抑制产生协同作用。To investigate the single and combined developmental toxicity of cadmium and nickel,mouse limb bud cell micromass culture was conducted to observe the effects of CdCl2 and NiCl2 on the proliferation and differentiation of mouse embryo limb bud cell.The results showed that CdCl2 at dosage of 0.1￣1.2 μg/ml and NiCl2 at dosage of 5.5￣27.5 μg/ml significantly inhibited the increase of cell number,CdCl2 at dosage of 0.4￣1.2 μg/ml and NiCl2 at dosage of 5.5￣27.5 μg/ml also reduced the number of cartilaginous foci,however,CdCl2 at dosage of 0.1 μg/ml and 0.2 μg/ml could increase the number of cartilaginous foci.These two metal ions in combination could affect the proliferation and differentiation of mouse embryo limb bud cell by adding together their inhibitory effects and potentiating the inhibition respectively.It is suggested that Cd and Ni in combination exhibited their effects on the inhibition of proliferation and differentiation of mouse embryo limb bud cell in a synergistic way.

关 键 词：镉 镍 胚胎肢芽细胞 增殖 分化 

分 类 号：R135.1[医药卫生—劳动卫生] R992[医药卫生—公共卫生与预防医学]