鸭副粘病毒WF_(01) D株F基因的测序与蛋白特性分析  

Sequencing and protein analysis of F gene of WF_(01) D from duck

作　　者：胡元庆[1] 张训海[1] 路振香[1] 金光明[1] 

机构地区：[1]家禽疫病防控监测安徽省重点实验室,安徽凤阳233100

出　　处：《中国微生态学杂志》2009年第5期399-402,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(30671556);安徽省教育厅自然科学基金一般项目(KJ2007B298)共同资助

摘　　要：用鸭副粘病毒凤阳分离株WF01 D作9～10日龄SPF鸡胚的尿囊腔接种，成功增殖了该病毒，采用一步法RT-PCR技术扩增WF01 D病毒的F基因，获得了1条长约1．7kb的特异性条带。用PCR产物直接测序。测序结果表明，扩增片段大小为1782bp，含有1个1662bp的开放性阅读框，编码554个氨基酸。核苷酸同源性分析表明：WF01 D株与国内外其他NDVF基因的同源性为84．5％～97．0％，其中与国内标准强毒株F48E9的同源性为86．6％，说明WF01 D与国内外的传统毒株有较大变异。与Taiwan95株和J株的同源性为93．6％和97．0％，说明WF01 D与Taiwan95株和J株亲缘关系较近，具有较高的相似性。F蛋白裂解位点的氨基酸顺序为112Arg-Arg-Gln-Lys-Arg-Phe117，表明为NDV的强毒株。蛋白疏水性和抗原性分析表明与标准强毒F48E9株相比没有太大的变异。The ND virus of WF01D was grown in 10-day-old SPF embrocated eggs. The F gene of Fengyang strain WF01 D of duck was amplified by one step RT-PCR. The sequencing analysis showed that the sequence of the F gene spanned 1782 bp including an ORF of 1662 bp and encoded a protein of 554 amino acids. The homology between WFol D and the NDV strain isolates inside and outside China was from 84.5% to 97.0%. The WF01 D F gene＇s nucleotide sequence shared 86.6% homology with that of the NDV strain F48E9. The ORF of WF01D F gene shared 93.6% and 97.0% nucleotide homology with that of the NDV strain Taiwan95 and J respectively. The results indicated the mutation on the F gene had varied largely,compared with classic NDV. The deduced amino acid sequences near cleavage site of F0 proteins showed a 112Arg-Arg-Gln-Lys-Arg-Phell7 motif, comforming well with that of the virulent strains. The analysis of WF01 D F protein hydrophobicity and epitope showed that, WF0, D strain was not a variation, compared with the standard strain F48E9.

关 键 词：鸭副粘病毒 F基因 序列测定 病毒毒力 同源性分析 

分 类 号：R373.16[医药卫生—病原生物学]