植物乳酸杆菌CGMCC NO.1258表层黏附蛋白同致病性大肠埃希菌竞争黏附肠上皮细胞的研究  

Study of the surface adhesive protein of Lactobacillus plantarum CGMCC NO.1258 on adhering to the gut epithelial cell by competing with the pathogenic Escherichia coli

作　　者：沈通一[1] 张明[1] 张鹏[1] 陈红旗[1] 周玉坤[1] 杭晓敏 秦环龙[1] 

机构地区：[1]上海交通大学附属第六人民医院外科,上海200233 [2]上海交大昂立微生物研究所,上海200233

出　　处：《中国微生态学杂志》2009年第5期403-406,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(30672044)

摘　　要：目的了解植物乳酸杆菌CGMCC NO.1258表层黏附蛋白(IMP2)在同肠上皮细胞(Caco-2)黏附作用中的机制。方法在制备和鉴定IMP2的多克隆抗体的基础上,同EPEC进行竞争黏附肠上皮细胞中,采用体外抗体阻断的方法检测IMP2对植物乳酸菌CGMCC NO.1258黏附作用的影响。结果多克隆抗体能够有效抑制植物乳酸菌CGMCC NO.1258对肠上皮细胞的黏附,并且导致EPEC对Caco-2细胞黏附的增加。结论IMP2在植物乳酸杆菌CGMCC NO.1258同肠上皮细胞黏附中发挥着重要的作用。Objective To investigate the effects and mechanism of the surface adhesive protein of Lactobacillus plantarum CGMCC NO. 1258 on adhering to the gut epithelial cell. Method On the basis of the preparation and identification of the polyeional antibody of IMP2, the role of IMP2 in the adhesion of Lactobacillus plantarum CGMCC NO. 1258 was tested by blocking the protein with the antibody in the competitive adhesion to the gut epithelial cell with the EPEC. Result The adhering role of the Lactobacillus plantarum CGMCC NO. 1258 was inhibited by the polyclonal antibody, which resulted in the increase of the adhesion of the EPEC to the epithelial cell. Conclusion IMP2 played important roles in the adhesion of Lactobacillus plantarum CGMCC NO. 1258 to the gut epithelial cell.

关 键 词：植物乳酸菌 表层黏附蛋白 多克隆抗体 

分 类 号：R378.992[医药卫生—病原生物学]