双歧杆菌表达ETEC CFA/I的免疫电镜定位分析  

Analysis of ETEC CFA/I expressed in bifidobacterium by immuno-electron microscope

作　　者：刘革力[1] 冉丹霞[1] 王荣荣[1] 郝亚宁[1] 宋方洲[1] 马永平[1] 

机构地区：[1]重庆医科大学生物化学与分子生物学教研室分子医学与肿瘤研究中心,重庆400016

出　　处：《中国微生态学杂志》2009年第5期415-416,共2页Chinese Journal of Microecology

基　　金：国家自然科学基金项目资助(30200244)

摘　　要：目的研究双歧杆菌表达的CFA／I在细胞中的分布。方法将培养8～10h的重组双歧杆菌固定后制作电镜切片，镜检合格后用镍网捞取3-5个切片与金标抗体作用，镜检CFA／I在双歧杆菌中的表达分布。结果自制的胶体金颗粒均匀，与抗体结合后能够示踪CFA／I在双歧杆菌中的表达分布。结论双歧杆菌表达的CFA／I主要集中在靠近细胞膜的周质腔一侧，利于向外分泌和释放。Objective To determine CFA/I expression area in bifutobacterium. Method After cultured 8 - 10 hours, the section of recombinant bifidobacterium was prepared for electron microscopy and then for reaction with golden labeled anti-CFA/I antibody. The resulted products were examined with H-7500 electron microscope. Result The golden la- beled antibody could react with CFA/I expressed in bifidobacterium. Conclusion Golden labeled CFA/I was found in the area of periplasmic space,which was favorable to CFA/I excretion as an oral live vaccine antigen.

关 键 词：双歧杆菌 口服疫苗 CFA/I 免疫电镜 

分 类 号：Q78[生物学—分子生物学]