量子点快速检测弓形虫IgG抗体方法的建立及初步应用  

Establishment and application of quantum dots for TOXO IgG detection

作　　者：李玲[1] 刘琳琳[1] 蒲晓允[1] 纽琴[1] 余娟春[1] 

机构地区：[1]第三军医大学新桥医院检验科,重庆400037

出　　处：《第三军医大学学报》2009年第11期1057-1059,共3页Journal of Third Military Medical University

基　　金：重庆市人口和计划生育委员会资助项目(06204)~~

摘　　要：目的建立一种简便、快速的人弓形虫(Toxoplasma,TOXO)IgG抗体检测新方法,并在临床初步应用。方法应用TOXO抗原包被羧基磁珠作为固相载体,量子点标记羊抗人IgG作为检测抗体,建立TOXO IgG检测方法,同时优化检测条件;对255份临床血清标本进行了检测,并与意大利DiaSorin TOXO IgG ELISA试剂进行比较。结果检测最佳条件为:抗原包被浓度100μg/ml,量子点标记二抗工作浓度为1∶200,待测血清稀释度为1∶100。本方法的诊断敏感性、特异性分别为93.3%、96.7%,与进口试剂盒的总符合率为96.5%。结论基于量子点标记及免疫磁珠技术检测TOXOIgG,操作简便、快速,具有较高的特异性、敏感性和有较好的临床应用价值。Objective To establish a new method for the detection of anti-Toxoplasma IgG antibodies （TOXO IgG） in serum and to evaluate its preliminary significance in clinical application. Methods The immunomagnetic beads were prepared by coating Toxoplasma antigen onto the surfaces of magnetic microspheres. The purified conjugate of goat anti-human IgG labeled with QDs was used as the second antibody. Then the TOXO IgG in the serum samples were detected and then the detection conditions were optimized. The serum of 255 pregnant women was assayed with this new method. The results were analyzed and compared with those by the DiaSorin TOXO IgG ELISA kit made in Italy. Results The best test conditions were determined as follows： the magnetic microspheres coated by Toxoplasma antigen at the concentration of 100 μg/ml, the working dilution of QDs-labeled rabbit anti-mice IgG at 1：200, and dilution of serum samples at 1： 100. The sensitivity and specificity were 93.3% and 96.7% , respectively. The coincidence rate with the imported kit reached as high as 96.5%. Conclusion The method established in this study is of easy operation, fast reaction, and reliable result, and may be of potential value in clinical application.

关 键 词：量子点 免疫磁珠 弓形虫 

分 类 号：R382.5[医药卫生—医学寄生虫学] R392.11[医药卫生—基础医学]