阿司匹林与DNA相互作用的光谱研究  

Study on the Interaction between Aspirin and DNA by Spectrometric Method

作　　者：刘炜[1] 毕和平[1] 张连华[1] 汤颖[2] 陈光英[1] 

机构地区：[1]海南师范大学化学与化工学院海南省热带药用植物化学重点实验室,海南海口571158 [2]西安石油大学化学化工学院,陕西西安710065

出　　处：《安徽农业科学》2009年第15期6837-6838,共2页Journal of Anhui Agricultural Sciences

基　　金：海南省重点科技项目(070207);海南师范大学博士启动基金资助项目(103307);海南省教育厅项目(Hjkj2009-39);海南省有机化学重点学科开放基金

摘　　要：[目的]研究阿司匹林(ASP)与DNA相互作用的机理。[方法]在pH值为4.5的Tris-HCl缓冲溶液体系中,应用荧光光谱法和紫外光谱技术研究ASP和DNA分子间的相互作用;利用Stern-Volmer方程探讨DNA对阿司匹林的荧光猝灭机制。[结果]DNA与ASP之间存在较强的相互作用,25℃时结合常数为2.17×106L/mol,结合位点数为1.773 5,结合方式是ASP通过嵌入结合的方式与DNA结合。[结论]该研究可为进一步研究ASP的药理活性提供重要信息。[ Objective]The research aimed to study the interaction mechanism between aspirin and DNA. [ Method]The interaction of aspirin and DNA was studied using UV spectrometry and fluorescence spectrometry. The quenching mechanism of fluorescence of aspirin by DNA was discussed through Stem- Volzner equation. [ Ressult]The results showed that there were strong interaction between aspirin and DNA. The binding constant was 2.17 × 10^6 L/mol at 25 ℃.The nmnber of binding sites was 1.773 5. And aspirin binded with DNA in a mode of intewalative binding with the result of IN - VIS spectrum. [ Conclusion]This study could provide important information for further study about the pharmacological, activity of aspirin.

关 键 词：阿司匹林 DNA 荧光猝灭 机制 

分 类 号：O657.3[理学—分析化学]