RAPD分子标记技术在甜高粱基因组研究中的应用  

Application of RAPD Molecular Marker Technology in Studying Sweet Sorghum Genome

作　　者：李南珠 李玥莹[2] 

机构地区：[1]沈阳市中医药学校,辽宁沈阳110300 [2]沈阳师范大学化学与生命科学学院,辽宁沈阳110034

出　　处：《安徽农业科学》2009年第15期6881-6883,6886,共4页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅重点实验室资助项目(20060806);公益性行业(农业)科研专项(nyhyzx07-011-03)

摘　　要：[目的]应用RAPD技术对甜高粱丝黑穗病基因进行分子标记研究。[方法]以抗病亲本7050B与感病亲本TX622B杂交后的F2代以及抗病甜高粱品种8113和感病甜高粱品种8101为试材,用CTAB法提取DNA,用RAPD分子标记技术对其DNA进行多态性扩增与初步分析,同时对琼脂糖凝胶电泳检测体系进行优化。[结果]CTAB法适宜提取DNA。在对抗病亲本7050B与感病亲本TX622B杂交后的F2代进行RAPD标记时,用60个引物进行筛选,其中27个引物扩增出了多态性谱带;应用20个具有多态性扩增谱带的引物对抗病甜高粱品种8113和感病甜高粱品种8101进行RAPD分析时,共有7个引物扩增出了差异谱带,分别为S56、S66S、67、S70、S72、S75S、78。[结论]该研究为甜高粱优良品种的培育提供科学基础。[ Objective] The study aimed to research the molecular marker of head smut gene of sweet sorghum by RAPD technology. [ Method] With the F2 from cross between the resistant parent 7050B and the susceptible parent TX622B and the sweet sorghum resistant variety 8113 and susceptible variety 8101 as the tested materials, their DNAs were extracted by CT_AB method. RAPD molecular marker technology was used to make the polymorphism amplification and preliminary analysis on their DNA and the detection system of agarese gel electmphomsis were also optimized. [ Result ] CrAB method was suitable to extract DNA. When F2 from cross between the resistant parent 7050B and the susceptible parent TX622B was made for RAPD molecular marker, 60 random primers were used to screen and 27 primers amplified out the polymorphism bands. When 20 primers with polymorphism amplification bands were used to make the RAPD analysis on the sweet sorghum resistant variety 8113 and susceptible variety 8101, there were 7 primers that amplified out the difference bands, being S56, S66, S67, S70, S72, S75 and S78 resp. [ Conclusion] This study provided the scientific foundation for breeding of good varieties of sweet sorghum.

关 键 词：甜高粱 丝黑穗病 CATB法 RAPD分子标记 

分 类 号：S514[农业科学—作物学]