感染性脑损伤机制和脑保护作用的研究  

The mechanism and neuroprotective effect of infective brain injury induced by Pertussis Bacilli

作　　者：陈立华[1] 杨于嘉[2] 曹美鸿[1] 马建荣[1] 陈翔[2] 陶永光[2] 

机构地区：[1]湖南医科大学湘雅医院神经外科,长沙410008 [2]湖南医科大学湘雅医院儿科,长沙410008

出　　处：《中华实验外科杂志》1998年第4期373-375,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(No.39470233)

摘　　要：目的探讨感染性脑损伤的发生机制及N-甲基-D-天冬氨酸(NMDA)受体非竞争性拮抗剂和Ca^(2+)通道阻滞剂对脑损伤的保护作用。方法采用百日咳菌液诱导感染性脑损伤模型,Fura-2/AM负载突触体,测定突触体胞浆内[Ca^(2+)]i变化及MK-801和尼莫地平对其变化的影响。结果突触体胞浆中[Ca^(2+)]i在注菌后30分钟即已明显升高,随脑损伤时间的延长,[Ca^(2+)]i持续进行性升高,尼莫地平治疗后,突触体胞浆内[Ca^(2+)]i明显下降;MK-801预处理后4小时和24小时组突触体胞浆内[Ca^(2+)]i亦明显降低(P<0.05)。结论感染性脑损伤的发生与神经细胞膜上电压依赖性Ca^(2+)通道开放和NMDA受体依赖性迟发性钙离子内流引起的钙离子超载密切相关,MK-801和尼莫地平通过缓解细胞内钙离子超载,减轻感染性脑损伤。Objective To study the mechanism of infective brain injury induced by Pertussis Bacilli (PB), and the neuroprotective effect of antagonists of Noncompetition NMDA receptor (MK-801) and antagonist of Ca^(2+)channels on the neuron. Methods The infective brain injury in SD rats was induced by injection of PB. Cytosolic free calcium ([Ca^(2+)] i) was determined in calcium fluorescent indication Fura2/AM loaded neuronal synaptosomes by using spectroflurophotometer. MK-801 was pretreated by injection of at 48th h and 24th h before bran injury by PB, nimodipine, calcium channel antagonist, was also administered after infective brain injury induced by PB to observe the influence on [Ca^(2+)]i. Results The level of [Ca^(2+)] i within neuronal synaptosomes was obviously increased 30 min after PB injection, and progressively elevated with the continuance of infective brain injury as compared with that in control group. After the treatment of nimodipine, [Ca^(2+)] i level was remarkably decreased (P<0.05). Pretreatment of MK—801 could significantly lower [Ca^(2+)] i level at 4th and 24h in PB group (P<0.05). Conclusion Ca^(2+)overloaded was an important factor leading to infective brain injury. Nimodipine treatment and MK-801 pretreatment could inhibit the inflow of Ca^(2+) into the neurons, and then reduce the neuronal damage after infective brain injury.

关 键 词：颅脑损伤 钙离子 尼莫地平 感染性 药物疗法 

分 类 号：R651.150.5[医药卫生—外科学]