pcDNA3.0-SV40T重组表达质粒的构建及鉴定  

Construction and Indentification of the Recombinant Expression Plasmid pcDNA3.0-SV40T

作　　者：李融[1] 赵国琦[1] 韩庆广[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《家畜生态学报》2009年第2期81-83,105,共4页Journal of Domestic Animal Ecology

摘　　要：选用pGEM-SV40 T质粒经XhoI单酶切获得SV40 T基因片段,并将其插入pcDNA3.0载体,构建重组质粒pcDNA3.0-SV40 T,为建立永生化细胞系奠定基础。结果表明,经酶切鉴定证实,SV40T基因片段已经成功插入载体中。构建的SV40病毒T抗原重组质粒为利用SV40T抗原进行真核细胞研究提供了稳定、可靠的分子工具。To design and construct recombinant expression plasmid pcDNA3.0--SV40T for the construction of immortalization cell lines, pGEM--SV40T plasmid was digested by restriction enzyme XhoI to harvest the SV40T DNA fragment. DNA fragment was inserted to the vector pcDNA3.0 by identification of restriction enzyme digestion. The results showed that the recombinant plasmid pcDNA3.0--SV40T will be a stable and valuable molecular tool~ for eukaryocyte study. SV40T gene can speed up the transformation of cell growth rate and at the same time be able to retain many of its original cell differentiation phenotype, especially in the slow proliferation of cells in vitro, the use of the antigen gene SV40T. The establishment of immortalized cell lines is of great significance.

关 键 词：SV40病毒 T抗原 重组质粒 永生化 

分 类 号：S811.5[农业科学—畜牧学]