布地奈德对A549细胞胸腺基质淋巴细胞生成素表达作用的研究  

Effect of budesonide on the expression of thymic stromal lymphopoietin in A549 cells

作　　者：陈真[1] 杜强[1] 周林福[1] 史莹[1] 殷凯生[1] 

机构地区：[1]南京医科大学第一附属医院呼吸科,江苏南京210029

出　　处：《现代生物医学进展》2009年第10期1820-1823,F0002,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30400191;30570797);江苏省"六大人才高峰"项目(06B035);江苏省医学重点人才项目(RC2007043)

摘　　要：目的:观察细胞因子刺激气道上皮细胞胸腺基质淋巴细胞生成素(TSLP)表达是否涉及核因子κB(NF-κB),并探讨糖皮质激素布地奈德对气道上皮细胞TSLP表达和NF-κB核转位的影响。方法:A549细胞与细胞因子白介素1β(IL-1β)、白介素4(IL-4)和布地奈德共同孵育,以不加任何细胞因子或布地奈德培养的A549细胞为对照组,采用RT-PCR方法测定TSLP mRNA表达,细胞免疫荧光方法检测TSLP和NF-κB的表达情况。结果:与对照组比较,IL-1β(10ng/ml)及IL-4(10ng/ml)显著刺激A549细胞TSLP mRNA表达,且NF-κB(p65)核转位增加(均P<0.05)。布地奈德干预后TSLP mRNA的表达和NF-κB(p65)的核转位显著减少(P<0.05)。结论:细胞因子促进气道上皮细胞诱导性表达TSLP与NF-κB激活有关,抑制TSLP表达和NF-κB激活可能是布地奈德治疗哮喘的重要机制。Objective： To investigate whether nuclear factor κB （NF-κB） is involved in the cytokine-induced expression of thymic stromal lymphopoietin （TSLP） and to explore the effect of budesonide on them. Methods： A549 cells were cultured with cytokines, interleukin1β （IL-1β） and IL-4, and budesonide （0.5 mg/ml）. The control group： A549 cells were maintained without cytokines and drugs. The expressions of TSLP mRNA were detected by RT-PCR and the nuclear translocations of NF-κB （p65） were detected by immunofluorescence assays. Results： Compared with the control group, the expression ofTSLP mRNA and nuclear p65 were significantly increased in A549 cells stimulated with IL-1β, IL-4 or both of them （all P〈0.05）, whereas that were greatly abolished in A549 cells pretreated with budesonide （P〈0.05）. Conclusions： Cytokines enhance the inducible expression of TSLP in bronchial epithelial cells associated with NF-κB activation. Inhibition of NF-κB activation and TSLP expression might be an essential therapeutic mechanism of budesonide for asthma therapy.

关 键 词：胸腺基质淋巴细胞生成素 A549 布地奈德 NF—κB 

分 类 号：R562.25[医药卫生—呼吸系统]