海洋细菌Cellulophaga sp.QY201产内切纤维素酶发酵条件的研究  

Study on Fermentation Conditions of Marine Bacterium Cellulophaga sp. QY201 for Endoglucanase production

作　　者：苏贝[1] 韩峰[1] 于文功[1] 

机构地区：[1]中国海洋大学医药学院海洋药物教育部重点实验室,山东青岛266003

出　　处：《现代生物医学进展》2009年第10期1874-1877,共4页Progress in Modern Biomedicine

摘　　要：本文对海洋细菌Cellulophaga sp.QY201产内切纤维素酶的发酵条件进行了研究。研究结果表明,该菌株最佳产酶的液体培养基组成为(w/v):CMCNa0.5%,CaSein0.3%,NaCl3%,MgSO4·7H2O0.3%,Na2HPO40.15%,NaH2PO40.1%,pH=7.0;最佳培养条件为:500mL三角瓶装液150mL,温度为28℃,转速为100r/min,发酵时间为36h。优化后发酵上清液的内切纤维素酶活力可达到7.85U/mL,比优化前提高了2.5倍。该菌株产酶发酵条件的研究,为内切纤维素酶的大规模制备及应用奠定了基础。The fermentation conditions of marine bacterium Cellulophaga sp. QY201 for endoglucanase production were investigated in this paper. The results showed that the optimal liquid fermentation medium was （w/v）： CMCNa 0.5 %, CaSein 0.3 %, NaCl 3 %, MgSO4·7H2O 0.3 %, Na2HPO4 0.15 %, NaH2PO4 0.1%, and pH=7.0. After fermentation for 36 h at 100 r/min and 28 ℃, the yield of endoglucanase was up to 7.85 U/mL, which was 2.5 times higher than that under the original conditions. All of this lays a solid foundation for large-scale preparation and application of endoglucanase.

关 键 词：海洋细菌 Cellulophaga sp.QY201 内切纤维素酶 发酵条件优化 

分 类 号：Q-331[生物学]