不同比例脱钙骨基质颗粒和骨水泥复合物修复兔股骨骨缺损  

Decalcified bone matrix and bone cement compound at various proportions in repairing rabbit femoral defect

作　　者：杜尧[1] 柯珍勇[1] 

机构地区：[1]重庆医科大学附属第二医院骨科,重庆市400010

出　　处：《中国组织工程研究与临床康复》2009年第21期4064-4068,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:新近研究表明骨水泥诱导成骨能力较差、在体内降解过慢,其单独应用的效果并不理想。因此,需要对其进行改型,希望研制出一种能克服上述缺点的新型材料运用于临床。目的:观察脱钙骨基质颗粒、丙烯酸树脂骨水泥复合物填充修复股骨骨缺损的能力,从而确定该复合材料的最佳配方。设计、时间及地点:随机对照动物实验,于2008-05/09在重庆医科大学动物实验中心完成。材料:将脱钙骨基质颗粒和丙烯酸树脂骨水泥按不同质量比例(2∶8,3∶7,4∶6,5∶5,6∶4)构成复合材料。方法:在新西兰大白兔双侧股骨制备骨缺损填充模型,将各比例复合材料植入骨缺损处,以单纯丙烯酸树脂骨水泥材料作为对照。主要观察指标:对复合材料和单纯材料进行扫描电镜观察及生物力学测试。在术后4,8,12周时进行大体标本观察、组织病理学、X射线片观察,比较其修复填充骨缺损的能力。结果:脱钙骨基质颗粒与丙烯酸树脂骨水泥质量比在3∶7～6∶4的范围内,复合材料中存在较多100μm以上的裂隙,当质量比小于3∶7时,材料内部的大部分间隙<100μm,质量比大于6∶4时两种材料不能有效地凝固在一起。随质量比的增加,材料抗压极限强度递减,各组数据经方差分析,差异具有显著性意义(P<0.05)。在各时间点大体标本观察、组织病理学、X射线片观察显示骨缺损填充部位均有不同程度新骨形成。12周时脱钙骨基质颗粒与丙烯酸树脂骨水泥质量比在4∶6时骨结合率最高。结论:随着脱钙骨基质颗粒质量比的增加,孔隙越丰富而材料力学强度逐渐降低。脱钙骨基质颗粒与丙烯酸树脂骨水泥质量比为4∶6时修复低承重部位松质骨的骨缺损效果较好。BACKGROUND： BACKGROUND： Present studies have shown that bone cement have less osteoinduction and slow degradation in the body, so the effect of alone application is not satisfactory. Therefore, people have modified them, hoping to research a new materials that can overcome all shortcomings above-mentioned. OBJECTIVE： To investigate the ability of decalcified bone matrix （DBM） and acrylic bone cement （ABC） compound in filling and repairing bone defect, and to determine the best component proportion of the composition. DESIGN, TIME AND SETTING： Randomized controlled animal experiments were performed at the Experimental Animal Center, Chongqing Medical University from May to September 2008. MATERIALS： ABC and DBM were prepared into compound material at various proportions （2：8, 3：7, 4：6, 5：5, 6：4）. METHODS： The animal models of bilateral femur bone defects and fillers were established in the New Zealand White rabbits. The complex material was implanted on the bone defect; the ABC was used as blank control group. MAIN OUTCOME MEASURES： The complex and alone materials were examined by biomecbanics and scan electron microscopes. Gross observation, histopathological and X-ray test were used at 4, 8, 12 weeks after operation respectively to observe the repaired and filled status of bone defect. RESULTS： DBM composite quality of the scope of 3：7 6：4, the composite materials that existed in more cracks more than 100 ; m, when the DBM was less than 3：7, the material inside the aperture and the majority of material was similar to ABC, mass ratio greater than 6：4 when the DBM and the ABC could not effectively together solidification. When the mass ratio was increased, which matrix particles of demineralized bone and ABC, the compressive ultimate strength of composite materials was less than pure ABC （P 〈 0.05）. After observing the result above at different time points, we found the new bone to form on the bone of defected and filled. On the twelfth week osseointegr

关 键 词：脱钙骨基质颗粒 骨水泥 复合材料 骨形成蛋白 骨质疏松 骨结合率 

分 类 号：R318[医药卫生—生物医学工程]