左归复方对MKR小鼠血糖血脂及炎症因子CRP、IL-6、TNF-α的影响  

Effects of Zuogui decoction on regulation of glucose-lipid metabolism and inflammatory cytokines in MKR mice

作　　者：胡伟[1] 喻嵘[1] 成细华[2] 伍参荣[2] 李萍[3] 赵愈[3] 肖漫江[4] Jun-Li Liu 

机构地区：[1]湖南中医药大学2001级本硕班,湖南长沙410007 [2]湖南中医药大学中医内科学重点研究室,湖南长沙410007 [3]湖南中医药大学湘杏学院2005级,湖南长沙410007 [4]湖南中医药大学2006级研究生班,湖南长沙410007 [5]Fraser Laboratories, Department of Medicine, McGill University, Montreal, QC. 687 Pine Avenue West, H3A 1A1, Canada

出　　处：《湖南中医药大学学报》2009年第2期14-16,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(30400604);国家中医药管理局出国留学人员资助项目(国中医药发[2004]68号-1);湖南省杰出青年基金资助项目(08JJ1005)

摘　　要：目的观察左归复方对MKR小鼠血糖血脂代谢及血清CRP、IL-6、TNF-α水平的影响。方法以MKR小鼠为观察对象,用左归复方对其进行30 d灌胃治疗。采用电化学法检测血糖,全自动分析仪检测血脂和CRP,双抗体夹心ELISA法分别检测血清IL-6、TNF-α含量。结果给药30 d后,左归复方低、高剂量组,文迪雅组均有显著降低血糖作用,与给药前模型组比较,差异均有统计学意义(P<0.01);而各用药组的降血糖作用相当(P>0.05)。给药30 d后,各组血清TCHO、TG、HDL、LDL的含量变化组间无统计学差异(P>0.05);而左归复方低、高剂量组,文迪雅组中血清CRP、IL-6和TNF-α含量均低于模型组,差异有统计学意义(P<0.01,P<0.05);但用药组间比较差异无统计学意义(P>0.05)。结论左归复方有显著降低MKR鼠空腹血糖、CRP、IL-6、TNF-α含量的作用,可改善MKR鼠2型糖尿病的炎症反应。Objective To observe the effect of Zuogui decoction on the levels of blood glucose and lipid, CRP, IL-6 and TNF-α of serum in MKR mice. Methods 40 MKR mice were randomly divided into four groups. After gavage of Zuogui recipe 30 days, the levels of IL-6 and TNF-α were measured with double-antibody sandwich ELISA, the levels of CRP and lipids with automated analysis, and the blood glucose with electrochemical detection method. Results The effects of low-dose and high-dose of the decoction groups, and Avandia group were significant to lower blood glucose, compared with before administration in model group P〈 0.01; the effect in all treatment groups was similar（P〉0.05）; the volume of TCHO, TG, HDL and LDL in all groups were no significant difference （P〉0.05）; but the levels of CRP, IL-6 and TNF-α in the low-dose, high-dose and Avandia groups were lower than that in model group （P〈0.01 ,P〈0.05）; otherwise, compared with the Avandia group, the levels of CRP, IL-6 and TNF-α in the low-dose and high-dose groups were no significant difference （P〉0.05）. Conclusion Zuogui recipe is very effective to reduce blood glucose, and content of serum CRP, IL- 6 and TNF-α, and improve the reaction of inflammation.

关 键 词：2型糖尿病 炎症因子 左归复方 MKR小鼠 熟地 山茱萸 黄芪 

分 类 号：R285.8[医药卫生—中药学]