HPLC法测定药用百合中薯蓣皂苷元的含量  

Determination of diosigenin contents in medicinal Bulbus Lilii with HPLC

作　　者：史艳霞[1] 周日宝[1] 童巧珍[1] 任旻琼[1] 王珊[1] 王晓明 

机构地区：[1]湖南中医药大学药学院,湖南长沙410007 [2]湖南省林木无性系育种重点实验室,湖南长沙410004

出　　处：《湖南中医药大学学报》2009年第2期37-39,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家科技部"十一五"科技支撑计划资助项目(2006BAI06A11-06);湖南省中医药管理局资助项目(2008089)

摘　　要：目的用HPLC法测定不同来源百合中薯蓣皂苷元的含量。方法采用Phenomenex ODS(4.6 mm×250mm,5μm)色谱柱,乙腈-水梯度洗脱,流速为0.7 mL/min,进样量为20μL,检测波长为203 nm,柱温为30℃。结果薯蓣皂苷元的进样量在0.04～1.60μg范围内与峰面积呈良好的线性关系。平均回收率为98.2%,RSD为2.1%(n=6)。结论该法操作简便、准确、灵敏度高,可作为药用百合质量控制的定量方法。Objective To determine the diosgenin contents in Bulbus lain from different areas with RP-HPLC. Methods The RP-HPLC were with＇4.6 mm×250 mm, 5μm phenomenex ODS column, mobile phase of acetonitrile-water, 0.7 mL/min flow rate, 20 μL sample injection, 203nm wavelength and 30 ℃ temperature. Results The best linear correlation was the sample injection in the range of 0.04-1.60 μg and peak area（n=6）; and the average recoveries of diosgenin was 98.2%（RSD=2.1%,n=6）. Conclusion The methods is simple and accurate with a good sensitivity, which can be used for quality control of the medicinal Bulbus Lilii.

关 键 词：百合 薯蓣皂苷元 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]