不同剂量γ射线照射人肝细胞差异基因表达谱的研究  

STUDY ON DIFFERENTIAL TRANSCRIPTIONAL PROFILE IN HUMAN HEPATOCYTE EXPOSED TO DIFFERENT DOSES γ RAY

作　　者：李建国[1] 闻建华[1] 段志凯[1] 田宇[1] 王放[1] 左雅慧[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,太原030006

出　　处：《辐射防护》2009年第3期148-153,共6页Radiation Protection

基　　金："十一五基础科研"资助项目(项目编号:012060201)

摘　　要：利用基因芯片技术,对受不同剂量(0.5Gy、2Gy、4Gy)γ射线照射的人肝细胞和未受照射的人肝细胞基因差异表达谱进行了研究和分析。结果表明,在所分析的14000多条基因中,三个不同照射剂量共同差异表达的基因表达谱有284条,其中明显上调的差异表达基因有261条,明显下调的差异表达基因23条。主要涉及到以下几类型基因:与干扰素受体有关的基因;与线粒体调控有关的基因;与甲型肝炎病毒受体有关的基因;与细胞周期调控有关的基因;与激酶有关的基因;以及与锌指蛋白有关的基因等等。对4个表达上调基因HAVcr-1、HAVcr-2、MFTC、MOAP1和2个表达下调基因TRIP12和DCN进行了荧光定量RT-PCR验证,实验结果与基因芯片检验结果相一致。The study analyzed the differential transcriptional profile of normal human hepatic cell and human hepatic cell radiated with three different doses （0.5 Gy, 2 Gy,4 Gy γ ray） by gene chip tech- nique. The results showed that the whole differentially expressed genes of three different doses have 284 in 14112 human genes analyzed, in which 261 genes were up-regulated and 23 genes were down-regulated. These genes are mainly associated with interferon receptor, mitochondrial regulation, homo sapiens hepatitis A virus cellular receptor, cell cycle regulation, kinase and zinc finger protein etc. RT-PCR results indicated that up-regulated expression of gene HAVcr-1, HAVer-2, MFTC, MOAP1 and down-regulated expression of gene TRIP12, DCN are consistent with gene chip data.

关 键 词：Γ射线 肝细胞 基因芯片 差异表达基因 

分 类 号：R144[医药卫生—公共卫生与预防医学]